标题:Spectrum-Based Electrochemiluminescent Immunoassay with Ternary CdZnSe Nanocrystals as Labels
作者:Zhang, Xin; Tan, Xiao; Zhang, Bin; Miao, Wujian; Zou, Guizheng
作者机构:[Zhang, Xin; Tan, Xiao; Zhang, Bin; Zou, Guizheng] Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China.; [Miao, Wujian] Univ Southern 更多
通讯作者:Zou, Guizheng
通讯作者地址:[Zou, GZ]Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China.
来源:ANALYTICAL CHEMISTRY
出版年:2016
卷:88
期:13
页码:6947-6953
DOI:10.1021/acs.analchem.6b01821
摘要:Conventional electrochemiluminescence (ECL) research has been performed by detecting the total photons (i.e., the ECL intensity). Herein, systematic spectral exploration on the ECL of dual-stabilizers-capped ternary CdZnSe nanocrystals (NCs) and its sensing application were carried out on a homemade ECL spectral acquiring system. The ternary CdZnSe NCs could be repeatedly injected with electrons via some electrochemical ways and then result in strong cathodic ECL with the coupling of ammonium persulfate. ECL spectrum of the CdZnSe NCs was almost identical to corresponding photoluminescence spectrum, indicating that the excited states of CdZnSe NCs in ECL were essentially the same as those in photoluminescence. Importantly, after being labeled to the probe antibody (Ab(2)) of alpha-fetal protein (AFP) antigen, the ternary NCs in the Ab(2)INCs conjugates could preserve their ECL spectrum very well. A spectrum-based ECL immunoassay was consequently proposed with the CdZnSe NCs as ECL tags and AFP as target molecules. The limit of detection is 0.010 pg/mL, with a signal-to-noise (S/N) ratio of 3, indicating a sensitive ECL sensing strategy that was different from the conventional ones. This work might open a pathway to the spectrally resolved ECL analysis with even-higher S/N ratios than the fluorescent analysis.
收录类别:EI;SCOPUS;SCIE
WOS核心被引频次:23
Scopus被引频次:25
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84979072504&doi=10.1021%2facs.analchem.6b01821&partnerID=40&md5=2914b38cd9a7aa25153e48d7bcbc71a0
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