标题:Ultrasensitive eletrogenerated chemiluminescence immunoassay by magnetic nanobead amplification
作者:Li, M.;Sun, Y.;Chen, L.;Li, L.;Zou, G.;Zhang, X.;Jin, W.
作者机构:[Li, M] School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P.R., China;[ Sun, Y] Information Science and Engineering Sch 更多
通讯作者:Zou, GZ
通讯作者地址:[Zou, GZ]Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China.
来源:Electroanalysis
出版年:2010
卷:22
期:3
页码:333-337
DOI:10.1002/elan.200900351
关键词:Carcinoembryonic antigen;Electrogenerated chemiluminescence;Immunoassays;Luminescence;Magnetic nanobead
摘要:An ultrasensitive electrogenerated chemiluminescence (ECL) immunoassay was proposed by using magnetic nanobeads (MNBs) as the carrier of ECL labels for ECL emission amplification. Carcinoembryonic antigen (CEA) and MNBs were initially immobilized on a platform in 1:1 molar ratio via sandwich immunoreaction. Subsequently, the MNBs were released from the platform and labeled with Ru(bpy)_3~(2+) species. After the MNBs with Ru(bpy)_3~(2+) were immobilized on an Au electrode, ECL of the Ru(bpy)_(32{thorn}) was measured for CEA determination. A linear relation between the ECL intensity and CEA concentration was obtained in a range of 1 × 10~(-14) to 3 × 10~(-13) mol/L (2.0 to 60 pg/mL) with a limit of detection of 8.0 × 10~(-15) mol/L (1.6 pg/mL).
收录类别:SCOPUS;SCIE
WOS核心被引频次:16
Scopus被引频次:17
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-75849134714&doi=10.1002%2felan.200900351&partnerID=40&md5=f71485d112b3619427a20069b00a6d87
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