标题:Associations of saposin C, Src, and androgen receptor upregulate the expression and function of androgen receptor in human prostate cancer cells.
作者:Ding Y;Wang X;Xu A;Xu X;Tian K;Young CY;Yuan H
作者机构:[Ding, Y] Department of Biochemistry and Molecular Biology, School of Medicine, Shandong University, 44 Wenhua Xi Road, Jinan 250012, China, School of 更多
通讯作者:Yuan, HQ
通讯作者地址:[Yuan, HQ]Shandong Univ, Sch Med, Dept Biochem & Mol Biol, 44 Wenhua Xi Rd, Jinan 250012, Peoples R China.
来源:Journal of Cellular Biochemistry
出版年:2011
卷:112
期:3
页码:818-828
DOI:10.1002/jcb.22977
关键词:SAPOSIN C; ANDROGEN RECEPTOR; SRC; PROSTATE CARCINOMA CELLS
摘要:We previously demonstrated that ectopic expression of neurotrophic peptide (NP) derived from saposin C promotes androgen receptor (AR) expression and transactivation in human prostate cancer cells. This prompted us to investigate how NP or saposin C can function in cells. We constructed plasmids expressing saposin C or a chimeric peptide of a viral TAT transduction domain and saposin C (TAT-saposin C) with His-tag. Intracellular localization of saposin C and NP was predominantly shown in transfected cells, while TAT-saposin C was detected around membrane and in cytosol by immunofluorescence staining. Furthermore, induction of the AR expression and activation of the AR transcriptional function were observed in cells transfected with saposin C or TAT-saposin C, compared to control cells transfected with an empty plasmid. The effects of saposin C and TAT-saposin C on AR activity were examined in the presence of inhibitors of GPCR, MAPK1/2, and PI3K/Akt. Interestingly, we found that these inhibitors only affect AR activities in cells with TAT-saposin C expression but not with saposin C expression. Immunostaining images showed that co-localization of saposin C, Src, and the AR occurred in transfected cells. Physical interactions of saposin C/NP, Src, and the AR were then demonstrated by co-immunoprecipitation assays. Blockage of Src activity by specific inhibitor led to a decrease in the saposin C-mediated enhancement of AR transactivity, suggesting that intracellular expression of saposin C caused stimulation of AR expression and activity by associations with Src in LNCaP cells. This effect may not be mediated by GPCR.
收录类别:SCOPUS;SCIE
WOS核心被引频次:5
Scopus被引频次:5
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951796016&doi=10.1002%2fjcb.22977&partnerID=40&md5=1ea053667752458c55e1a93e9d64744d
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