标题：Enhancing Production of Alkaline Polygalacturonate Lyase from Bacillus subtilis by Fed-Batch Fermentation
作者：Zou, Mouyong; Guo, Fenfen; Li, Xuezhi; Zhao, Jian; Qu, Yinbo
作者机构：[Zou, Mouyong; Guo, Fenfen; Li, Xuezhi; Zhao, Jian; Qu, Yinbo] Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China.
通讯作者地址：[Li, XZ]Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China.
摘要：Alkaline polygalacturonate lyase (PGL, EC 126.96.36.199) is an enzyme used in many industries. We developed a fed-batch fermentation process that combines the enzymatic pretreatment of the carbon source with controlling the pH of the fermentative broth to enhance the PGL production from Bacillus subtilis 7-3-3 to decrease the production cost. Maintaining the fermentation broth at pH 6.5 prior to feeding with ammonia and at pH 6.0 after feeding significantly improved PGL activity (743.5 U mL(-1)) compared with the control (202.5 U mL(-1)). The average PGL productivity reached 19.6 U mL(-1) h(-1) after 38 h of fermentation. The crude PGL was suitable for environmentally friendly ramie enzymatic degumming.