标题:Enhancing Production of Alkaline Polygalacturonate Lyase from Bacillus subtilis by Fed-Batch Fermentation
作者:Zou, Mouyong; Guo, Fenfen; Li, Xuezhi; Zhao, Jian; Qu, Yinbo
作者机构:[Zou, Mouyong; Guo, Fenfen; Li, Xuezhi; Zhao, Jian; Qu, Yinbo] Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China.
通讯作者:Li, XZ
通讯作者地址:[Li, XZ]Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China.
来源:PLOS ONE
出版年:2014
卷:9
期:3
DOI:10.1371/journal.pone.0090392
摘要:Alkaline polygalacturonate lyase (PGL, EC 4.2.2.2) is an enzyme used in many industries. We developed a fed-batch fermentation process that combines the enzymatic pretreatment of the carbon source with controlling the pH of the fermentative broth to enhance the PGL production from Bacillus subtilis 7-3-3 to decrease the production cost. Maintaining the fermentation broth at pH 6.5 prior to feeding with ammonia and at pH 6.0 after feeding significantly improved PGL activity (743.5 U mL(-1)) compared with the control (202.5 U mL(-1)). The average PGL productivity reached 19.6 U mL(-1) h(-1) after 38 h of fermentation. The crude PGL was suitable for environmentally friendly ramie enzymatic degumming.
收录类别:SCOPUS;SCIE
WOS核心被引频次:5
Scopus被引频次:5
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84897372799&doi=10.1371%2fjournal.pone.0090392&partnerID=40&md5=2e763d26331e93c3a650f6a8a1667228
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