标题：Regeneration of annulus fibrosus tissue using a DAFM/PECUU-blended electrospun scaffold
作者：Liu C.; Xiao L.; Zhang Y.; Zhao Q.; Xu H.
作者机构：[Liu, C] Anhui Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China, Spine Research Center of Wannan Medical 更多
通讯作者地址：[Xu, H] Anhui Provincial Hospital, Cheeloo College of Medicine, Shandong UniversityChina;
来源：Journal of Biomaterials Science, Polymer Edition
关键词：Annulus fibrosus-derived stem cells (AFSC); coaxial electrospinning; decellularized annulus fibrosus matrix (DAFM); poly(ether carbonate urethane) urea (PECUU); tissue engineering
摘要：Low back pain and disc degeneration affect quality of life and imposes an enormous financial burden. Although annulus fibrosus (AF) tissue engineering provides an alternative therapeutic possibility in the treatment of degenerative intervertebral disc disease, it is restricted by the biochemical properties, organizational structure, and mechanical characteristics of the scaffold. The ideal scaffold should closely mimic the natural extracellular matrix (ECM) in structure and function for long-term stability and survival. Poly(ether carbonate urethane) urea (PECUU) can be electrospun into nanofibrous scaffolds to mimic ECM architecture with the appropriate mechanical properties. However, PECUU scaffolds lack the bioactivity of natural ECM. On the other hand, a decellularized annulus fibrosus matrix (DAFM) has good biocompatibility and biodegradability and has been shown to promote secretion of AF-related ECM. Herein, DAFM/PECUU-blended electrospun scaffolds were fabricated with the help of coaxial electrospinning technology for the first time. AF-derived stem cells were cultured on DAFM/PECUU electrospun scaffolds, and cellular metabolic activity, morphology, and gene expression assays as well as AF-related ECM synthesis were performed. The results showed that AF-derived stem cells proliferated well on the scaffolds. Gene expression and ECM secretion of collagen type I and II and aggrecan from AF-derived stem cells cultured on DAFM/PECUU electrospun scaffolds were higher than from those on PECUU fibrous scaffolds. Thus, DAFM/PECUU electrospun scaffolds are a potential candidate for AF tissue engineering applications. © 2020 Informa UK Limited, trading as Taylor & Francis Group.