标题：Dendritic cell costimulatory molecule expression and immunosuppressant intervention
作者：Yang X.-Y.; Yao Q.-C.; Wang W.; Qi M.; Hong X.-Y.; Liu C.-M.; Yin H.; Liu H.;等 更多 作者机构：[Yang, X.-Y] Department of Urology, Beijing Chaoyang Hospital of Capital Medical University, Beijing, 100020, China;[ Yao, Q.-C] Department of Critica 更多
通讯作者地址：[Zhang, X.-D] Department of Urology, Beijing Chaoyang Hospital of Capital Medical UniversityChina
来源：Chinese Journal of Tissue Engineering Research
关键词：Dendrites; Lymphocytes; Rapamycin; Tissue Engineering
摘要：BACKGROUND: Previous studies on immunosuppression and anti-rejection after organ transplantation mainly focused on effects of T lymphocytes-mediated immune response and immunosuppressive agents on T lymphocytes. Effects of dendritic cells were unclear. The manifestation and mechanism of immunosuppressive agent effects on dendritic cells are not identical. OBJECTIVE: To compare the effects of different immunosuppressive agents on expression and function of costimulatory molecules of dendritic cells, and to explore the mechanism of action of immunosuppressive agents. METHODS: 20 μg/L rapamycin, 0.04 mg/L mycophenolate, 10 μg/L tacrolimus and 1 mg/L cyclosporine A were separately added during bone marrow cells of C57BL/6 mice were differentiated into dendritic cells. RESULTS AND CONCLUSION: Flow cytometry results revealed that CD40 expression in each group: rapamycin < tacrolimus < cyclosporine A < mycophenolate mofetil (P < 0.01); CD86 expression: rapamycin < tacrolimus < cyclosporine A = mycophenolate mofetil (P < 0.01). There was no significant difference in CD80, CD11c and major histocompatibility complex II expression among groups (P > 0.05). One-way mixed lymphocyte reaction results displayed dendritic cell costimulatory T cell proliferation in each group: rapamycin < mycophenolate mofetil < tacrolimus = cyclosporine A (P < 0.05). The results confirmed that rapamycin, tacrolimus, cyclosporine A, and mycophenolate mofetil exerted immunosuppression by suppressing CD40 and CD86, especially rapamycin. Above medicines do not noticeably inhibit major histocompatibility complex II expression, but suppress T cell proliferation, and the inhibitory effect of rapamycin on cell proliferation is strongest. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All Rights Reserved.