标题： Packaging of RVFV pseudoviruses and establishment of a neutralization assay method.
作者： Li, Yuetao; Zhao, Yongkun; Wang, Cuiling; Zheng, Xuexing; Wang, Hualei; Gai, Weiwei; Jin, Hongli; Yan, Feihu; Qiu, Boning; Gao, Yuwei; Li, Nan; Yang, 更多 作者机构：[ Li, Yuetao; Li Y] College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China., Institute of Milit 更多
来源： Journal of veterinary science
DOI： jvs.2016.104 [pii]
摘要： Rift Valley fever (RVF) is an acute, febrile zoonotic disease that is caused by the RVF virus (RVFV). RVF is mainly prevalent on the Arabian Peninsula, the African continent, and several islands in the Indian Ocean near southeast Africa. RVFV has been classified by the World Organisation for Animal Health (OIE) as a category A pathogen. To avoid the biological safety concerns associated with use of the pathogen in RVFV neutralization assays, the present study explored and established an RVFV pseudovirus-based neutralization assay. This study specifically used the HIV lentiviral packaging system and RVFV structural proteins to successfully construct RVFV pseudoviruses. Electron microscopy observation and western blotting indicated that the size, structure, and shape of the packaged pseudoviruses were highly similar to those of HIV lentiviral vectors. Infection inhibition assay results showed that an antibody against RVFV inhibited the infective ability of the RVFV pseudoviruses, and an antibody neutralization assay for RVFV detection was then established. Therefore, this study successfully established a neutralization assay based on RVFV pseudoviruses and demonstrated that this method can be used to effectively evaluate antibody neutralization.