标题：Hypoxia-responsive miR-141–3p is involved in the progression of breast cancer via mediating the HMGB1/HIF-1α signaling pathway
作者：Sun S.; Ma J.; Xie P.; Wu Z.; Tian X.
作者机构：[Sun, S] Cheeloo College of Medicine, Shandong University, Jinan, Shandong Province, China, Department of Breast and Thyroid Surgery, Liaocheng People 更多
通讯作者地址：[Wu, Z] Department of Breast and Thyroid Surgery, Liaocheng People's Hospital, Department of Breast and Thyroid Surgery, Shandong Provincial Hospital, 更多
来源：Journal of Gene Medicine
关键词：breast cancer; HIF-1α; HMGB1; hypoxia; miR-141–3p
摘要：Background: Hypoxia-responsive miRs have been frequently reported in the growth of various malignant tumors. The present study aimed to investigate whether hypoxia-responsive miR-141–3p was implicated in the pathogenesis of breast cancer via mediating the high-mobility group box protein 1 (HMGB1)/hypoxia-inducible factor (HIF)-1α signaling pathway. Materials and methods: miRs expression profiling was filtrated by miR microarray assays. Gene and protein expression levels, respectively, were examined by a quantitative reverse transcriptase-polymerase chaion reaction and western blotting. Cell migration and invasion were analyzed using a transwell assay. Cell growth was determined using nude-mouse transplanted tumor experiments. Results: miR-141–3p was observed as a hypoxia-responsive miR in breast cancer. miR-141–3p was down-regulated in breast cancer specimens and could serve as an independent prognostic factor for predicting overall survival in breast cancer patients. In addition, the overexpression of miR-141–3p could inhibit hypoxia-induced cell migration and impede human breast cancer MDA-MB-231 cell growth in vivo. Mechanistically, the hypoxia-related HMGB1/HIF-1α signaling pathway might be a possible target of miR-141–3p with respect to preventing the development of breast cancer. Conclusions: Our finding provides a new mechanism by which miR-141–3p could prevent hypoxia-induced breast tumorigenesis via post-transcriptional repression of the HMGB1/HIF-1α signaling pathway. © 2020 The Authors. The Journal of Gene Medicine published by John Wiley & Sons Ltd