标题:Mechanism of Wnt/β-catenin signaling pathway in enhanced malignant phenotype of non-small cell lung cancer induced by anti-angiogenesis therapy
作者:Xiao-Xue Zhang;Ling-Ling Zhang;Huan-Lian Yang;Xiu-Wen Wang
作者机构:Shandong University School of Medicine, Qingdao, Shandong 250000, China;Department of 0ncology, Binzhou People’s Hospital, Binzh
来源:亚太热带医药杂志(英文版)
出版年:2016
期:1
页码:56-61
DOI:10.1016/j.apjtm.2015.12.012
关键词:Anti-angiogenesis;Wnt1;β-catenin;Epithelial mesenchymal transition;Proliferation and invasion
摘要:Objective: To study the mechanism of Wnt/β-catenin signaling pathway in the enhanced malignant phenotype of A549 cells of human non-small cell lung cancer induced by the anti-angiogenesis therapy. Methods: The siRNA technique was employed to inhibit the expression of vascular endothelial growth factor (VEGF) in A549 cells and simulate the clinical course of anti-angiogenesis therapy. Real-time PCR and western-blot were used to study the change in the expression of Wnt/β-catenin signaling molecules at the mRNA and protein level respectively, as well as the effect on the epithelial mesenchymal transition in A549 cells. The proliferation and invasion abilities of tumor cells were detected to discuss the mechanism of Wnt/β-catenin signaling pathway in the enhanced malignant phenotype of non-small cell lung cancer induced by the anti-angiogenesis therapy. Results: The specific siRNA could significantly inhibit the expression of VEGF in cells to simulate the anti-angiogenesis therapy. Under the action of 50nM VEGF siRNA, the proliferation ability of A549 significantly increased (P
资源类型:期刊论文
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