标题：Enhanced activity of intracellular agarase from a novel marine strain Agarivorans gilvus WH0801
作者：Gu, Wenxue; Chen, Yulin; Niu, Huina; Lu, Xiao; Mao, Xiangzhao; Du, Zongjun; Liu, Xinli
作者机构：[Gu, Wenxue; Liu, Xinli] Shandong Polytech Univ, Shandong Prov Key Lab Microbial Engn, 3501 Daxue Rd, Jinan 250353, Peoples R China.; [Chen, Yulin; 更多
会议名称：2nd International Conference on Chemical Engineering and Advanced Materials (CEAM 2012)
会议日期：JUL 13-15, 2012
来源：ADVANCES IN CHEMISTRY RESEARCH II, PTS 1-3
关键词：Agarivorans gilvus; agarase; optimization; Plackett-Burman design;; Box-Behnken design
摘要：A marine bacterium strain Agarivorans gilvus WH0801 with the efficient agar degradation ability isolated from fresh seaweed samples of Weihai coast was found to be potential in producing agarase. We studied on the optimal medium composition and culture conditions of Agarivorans gilvus WH0801 by statistical methods in shake flasks. First, several more important factors influencing agarase activity were selected by Plackett-Burman design. They are agar concentration, yeast extract concentration and seed age. Then the optimum levels of these three variables were further determined using Box-Behnken design. The highest agarase activity is obtained in the medium consisting of 2.49 g L-1 agar and 0.88 g L-1 yeast extract when the seed age is 25.64 h. The levels of other factors are 1 g L-1 peptone, 0.01 g L-1 ironic citrate at initial pH 7.0 and 28 degrees C. The whole optimization strategy results in the activity of agarase reaches 1.158 U mL(-1), which is about 6.2-fold increase compares with the control.