标题:Comprehensive analysis of prognostic biomarkers in lung adenocarcinoma based on aberrant lncRNA-miRNA-mRNA networks and Cox regression models
作者:Yao, Yan; Zhang, Tingting; Qi, Lingyu; Liu, Ruijuan; Liu, Gongxi; Wang, Jia; Song, Qi; Sun, Changgang
作者机构:[Yao, Yan; Song, Qi] Weifang Med Univ, Clin Med Coll, 7166 Baotong Western St, Weifang, Shandong, Peoples R China.; [Zhang, Tingting; Qi, Lingyu] Sh 更多
通讯作者:Sun, CG
通讯作者地址:[Sun, CG]Shandong Univ Tradit Chinese Med, Innovat Inst Chinese Med & Pharm, Jinan 250014, Shandong, Peoples R China.
来源:BIOSCIENCE REPORTS
出版年:2020
卷:40
期:1
DOI:10.1042/BSR20191554
关键词:Competing endogenous RNA network; Cox regression; Lung adenocarcinoma; Overall survival; Prognostic biomarker
摘要:Lung adenocarcinoma (LUAD) is the leading cause of cancer-related death worldwide, and its underlying mechanism remains unclear. Accumulating evidence has highlighted that long non-coding RNA (lncRNA) acts as competitive endogenous RNA (ceRNA) and plays an important role in the occurrence and development of LUAD. Here, we comprehensively analyzed and provided an overview of the lncRNAs, miRNAs, and mRNAs associated with LUAD from The Cancer Genome Atlas (TCGA) database. Then, differentially expressed lncRNAs (DElncRNA), miRNAs (DEmiRNA), and mRNAs (DEmRNA) were used to construct a lncRNA-miRNA-mRNA regulatory network according to interaction information from miR-code, TargetScan, miRTarBase, and miRDB. Finally, the RNAs of the network were analyzed for survival and submitted for Cox regression analysis to construct prognostic indicators. A total of 1123 DElncRNAs, 95 DEmiRNAs, and 2296 DEmRNAs were identified (vertical bar log(2)FoldChange vertical bar (FC) > 2 and false discovery rate (FDR) or adjusted P value < 0.01). The ceRNA network was established based on this and included 102 lncRNAs, 19 miRNAs, and 33 mRNAs. The DEmRNAs in the ceRNA network were found to be enriched in various cancer-related biological processes and pathways. We detected 22 lncRNAs, 12 mRNAs, and 1 miRNA in the ceRNA network that were significantly associated with the overall survival of patients with LUAD (P < 0.05). We established three prognostic prediction models and calculated the area under the 1,3,5-year curve (AUC) values of lncRNA, mRNA, and miRNA, respectively. Among them, the prognostic index (PI) of lncRNA showed good predictive ability which was 0.737, 0.702 and 0.671 respectively, and eight lncRNAs can be used as candidate prognostic biomarkers for LUAD. In conclusion, our study provides a new perspective on the prognosis and diagnosis of LUAD on a genome-wide basis, and develops independent prognostic biomarkers for LUAD.
收录类别:SCOPUS;SCIE
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85078816053&doi=10.1042%2fBSR20191554&partnerID=40&md5=f8f1a18ebbadf92c24f096bd1c90b582
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