标题:Toxic interaction between acid yellow 23 and trypsin: Spectroscopic methods coupled with molecular docking
作者:Wang, Jing; Liu, Rutao; Qin, Pengfei
作者机构:[Wang, Jing; Liu, Rutao; Qin, Pengfei] Shandong Univ, Sch Environm Sci & Engn, Shandong Key Lab Water Pollut Control & Resource, China Amer CRC Enviro 更多
通讯作者:Liu, RT
通讯作者地址:[Liu, RT]Shandong Univ, Sch Environm Sci & Engn, Shandong Key Lab Water Pollut Control & Resource, China Amer CRC Environm & Hlth, 27 Shanda S Rd, Jin 更多
来源:JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
出版年:2012
卷:26
期:9
页码:360-367
DOI:10.1002/jbt.21430
关键词:Trypsin; Acid Yellow 23; Multispectroscopic Techniques; Toxic; Evaluation; Molecular Modeling
摘要:Acid yellow 23 (AY23) is a pervasive azo dye used in many fields which is potentially harmful to the environment and human health. This paper studied the toxic effects of AY23 on trypsin by spectroscopic and molecular docking methods. The addition of AY23 effectively quenched the intrinsic fluorescence of trypsin via static quenching with association constants of K290,K = 3.67 x 105 L mol-1 and K310,K = 1.83 x 105 L mol-1. The calculated thermodynamic parameters conformed that AY23 binds to trypsin predominantly via electrostatic forces with one binding site. Conformational investigations indicated the skeletal structure of trypsin unfolded and the microenvironment of tryptophan changed with the addition of AY23. Molecular docking study showed that AY23 interacted with the His 57 and Lys 224 residue of trypsin and led to the inhibition of enzyme activity. This study offers a more comprehensive picture of AY23trypsin interaction and indicates their interaction may perform toxic effects within the organism. (c) 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:360367, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21430
收录类别:SCOPUS;SCIE
WOS核心被引频次:11
Scopus被引频次:11
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84866461512&doi=10.1002%2fjbt.21430&partnerID=40&md5=66294507a502233807ac596612f8e3df
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