标题：The overexpression of Rab9 promotes tumor progression regulated by XBP1 in breast cancer
作者：Liu, Yansong; Wang, Xin; Zhang, Zhonghua; Xiao, Bin; An, Baoming; Zhang, JUn
作者机构：[Liu, Yansong] Shandong Univ, Shandong Canc Hosp, Breast Dept, Jinan, Shandong, Peoples R China.; [Wang, Xin] Yinan Country Peoples Hosp, Breast Dep 更多
通讯作者地址：[Liu, YS]Shandong Univ, Shandong Canc Hosp, Dept Breast Surg, Jiyan Rd 440, Jinan 250000, Shandong, Peoples R China.
来源：ONCOTARGETS AND THERAPY
关键词：transcription factor; proliferation; MCF7; MDA-MB-231
摘要：Background: Rab9 is a small GTPase that localizes to the trans-Golgi Network (TGN) and late endosomes and is involved in the recycling of mannose-6-phosphate receptors (MPRs).; Materials and methods: To determine new treatment strategies for breast cancer and to elucidate the mechanism underlying the phenomenon, we investigated the effects of Rab9 in the human breast cancer cell lines MCF7 and MDA-MB-231.; Results: We observed that knockdown of Rab9 inhibited the survival and proliferation of MCF7 and MDA-MB-231 cells, whereas Rab9 overexpression facilitated cell survival and proliferation by inducing or suppressing apoptosis. These results were further confirmed by the Bax/Bcl-2 ratio in affected MCF7 and MDA-MB-231 cells, which demonstrated whether the mitochondrial apoptotic pathway was triggered. Furthermore, the AKT/PI3K pathway is implicated in cell growth and survival and Rab9 changed the expression and phosphorylation of PI3K signaling pathway members. XBP1 is a key regulator of Rab9 and further confirmed that Rab9 play important roles in breast cancer tumorigenesis.; Conclusion: These data suggest that Rab9 is a good candidate for a novel therapeutic strategy for the treatment of breast cancer.