标题：TNF-alpha Increases Bone Marrow Mesenchymal Stem Cell Migration to Ischemic Tissues
作者：Xiao, Qiong; Wang, Shi-kun; Tian, Hua; Xin, Li; Zou, Zhi-geng; Hu, Yan-lai; Chang, Cui-ming; Wang, Xue-ying; Yin, Qun-sheng; Zhang, 更多 作者机构：[Xiao, Qiong; Wang, Shi-kun; Tian, Hua; Xin, Li; Zou, Zhi-geng; Hu, Yan-lai; Wang, Xue-ying; Yin, Qun-sheng; Zhang, Xiang-hong; Wang, Li-yan] Shandong 更多
通讯作者地址：[Tian, H]Shandong Univ, Inst Anat & Histol & Embryol, Sch Med, Jinan 250012, Shandong, Peoples R China.
来源：CELL BIOCHEMISTRY AND BIOPHYSICS
关键词：Mesenchymal stem cells; Adhesion molecules; Inflammatory factors;; Vascular endothelial cells
摘要：The objective of this study was to analyze the influence of TNF-alpha on rat mesenchymal stem cells (MSCs) and to assess feasibility of MSC transplantation to repair ischemic injury. In this study, adhesion molecules and cell specific surface markers on MSCs were measured after exposure to different concentrations of TNF-alpha. MSCs stimulated with varying concentrations of TNF-alpha were cultured with aortic endothelial cells, and the adhesion rate was measured. MSCs were then stimulated with an optimum concentration of TNF-alpha as determined in vitro, and injected intravenously into rats with ischemic hind limb injury. The number of MSCs in muscle samples from the ischemic area was counted. The results showed that (1) TNF-alpha induced a concentration-dependent increase in VCAM-1 expression in MSCs, whereas the expression of L-selectin, ICAM-1 and VLA-4 did not change significantly. Expression of MSC-specific antigens was unchanged. (2) MSCs pretreated with 10 ng/ml TNF-alpha showed significantly increased adhesion to endothelial cells in vitro, and accumulated to a greater extent in the areas of ischemic damage in rat hind limbs. We were able to conclude that TNF-alpha has no effect on expression of MSC-specific markers, but can increase the expression of VCAM-1 on rat MSCs. Suitable concentrations of TNF-alpha can promote MSC adhesion to endothelial cells and migration to damaged tissue.