标题：Attenuation of hind-limb suspension-induced bone loss by curcumin is associated with reduced oxidative stress and increased vitamin D receptor expression
作者：Xin, M.; Yang, Y.; Zhang, D.; Wang, J.; Chen, S.; Zhou, D.
作者机构：[Xin, M.; Yang, Y.; Zhou, D.] Shandong Univ, Prov Hosp, Dept Orthopaed, Jinan 250021, Peoples R China.; [Zhang, D.] Shandong Univ, Dept Anaesthesiol 更多
通讯作者地址：[Zhou, D]Shandong Univ, Prov Hosp, Dept Orthopaed, 324 JingWu Rd, Jinan 250021, Peoples R China.
关键词：Microgravity; Orally; Rats; Reactive oxygen species; Rotary wall vessel; bioreactor
摘要：Treatment with curcumin attenuated modeled microgravity-induced bone loss, possibly through abating oxidative stress and activating vitamin D receptor. Curcumin might be an effective countermeasure for microgravity-induced bone loss but remains to be tested in humans.; Introduction Bone loss is one of the most important complications for human crewmembers who are exposed to long-term microgravity in space and also for bedridden people. The aim of the current study was to elucidate whether treatment with curcumin attenuated bone loss induced by microgravity.; Methods We used hind-limb suspension (HLS) and rotary wall vessel bioreactor (RWVB) to model microgravity in vivo and in vitro, respectively. We investigated the effects of curcumin consumption (40 mg kg(-1) body weight day(-1), via daily oral gavages) on Sprague-Dawley (SD) rats exposed to HLS for 6 weeks. Then, we investigated the effects of incubation with curcumin (4 mu M) on MC3T3-E1 and RAW264.7 cells cultured in RWVB.; Results Curcumin alleviated HLS-induced reduction of bone mineral density in tibiae and preserved bone structure in tibiae and mechanical strength in femurs. Curcumin alleviated HLS-induced oxidative stress marked by reduced malondialdehyde content and increased total sulfhydryl content in femurs. In cultured MC3T3-E1 cells, curcumin inhibited modeled microgravity-induced reactive oxygen species (ROS) formation and enhanced osteoblastic differentiation. In cultured RAW264.7 cells, curcumin reduced modeled microgravity-induced ROS formation and attenuated osteoclastogenesis. In addition, curcumin upregulated vitamin D receptor (VDR) expression in femurs of rats exposed to HLS and MC3T3-E1 cells exposed to modeled microgravity.; Conclusion Curcumin alleviated HLS-induced bone loss in rats, possibly via suppressing oxidative stress and upregulating VDR expression.