标题:Cloning, expression and cytotoxicity of granulin A, a novel polypeptide contained in human progranulin
作者:Wang, Xuan; Xu, Huanli; Chen, Xiaoliang; Tian, Ye; Wang, Fengshan; Lin, Xiukun
作者机构:[Wang, Xuan; Wang, Fengshan] Shandong Univ, Sch Pharmaceut Sci, Inst Biochem & Biotechnol Drugs, Jinan 250012, Shandong, Peoples R China.; [Wang, Xu 更多
通讯作者:Wang, FS;Lin, XK
通讯作者地址:[Wang, FS]Shandong Univ, Sch Pharmaceut Sci, Inst Biochem & Biotechnol Drugs, Jinan 250012, Shandong, Peoples R China;[Lin, XK]Capital Med Univ, Dept 更多
来源:BIOSCIENCE TRENDS
出版年:2016
卷:10
期:3
页码:181-187
DOI:10.5582/bst.2016.01036
关键词:Granulin A; cloning; expression; anticancer activity
摘要:In our previous study, we isolated an antitumor polypeptide, CS5931, from the sea squirt Ciona savignyi; it shares high homology with Ciona intestinalis Granulin A (GRN A). However, little is known about the anticancer effect of GRN A. In the present study, GRN A was cloned and expressed in the yeast Pichia pastoris. The polypeptide was purified to almost homogeneity using a Ni-NTA column. MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide] assay reveals that GRN A displays potent cytotoxicity to several human cancer cells. The polypeptide induces cell apoptosis as analyzed by morphological observation and flow cytometry assays. This study provides evidence that GRN A possesses potential to be developed as a novel anticancer agent.
收录类别:SCOPUS;SCIE
WOS核心被引频次:2
Scopus被引频次:2
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84980377986&doi=10.5582%2fbst.2016.01036&partnerID=40&md5=d5d4808682780fdcf1aa4c7cc3cf6663
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