标题：Assessing Atrazine-Induced Toxicities in Bufo bufo gargarizans Cantor
作者：Sai, Linlin; Wu, Qingbo; Qu, Binpeng; Bo, Cunxiang; Yu, Gongchang; Jia, Qiang; Xie, Lin; Li, Yuezhong; Guo, Qiming; Ng, Jack C.; P 更多 作者机构：[Sai, Linlin; Li, Yuezhong] Shandong Univ, Sch Life Sci, State Key Lab Microbial Technol, Jinan 250100, Shandong, Peoples R China.; [Sai, Linlin; Bo 更多
通讯作者地址：[Guo, QM]Shandong Acad Occupat Hlth & Occupat Med, Dept Toxicol, Jinan 250002, Shandong, Peoples R China.
来源：BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
关键词：Herbicide; Toad; Endocrine disruption; Metamorphosis; Testis;; Histopathology
摘要：Atrazine (AZ), a widely used herbicide has drawn attentions for its potential impacts on amphibians. This study aims to investigate the toxicity of AZ in Bufo bufo gargarizans Cantor (B. bufo gargarizans), a species of toad commonly found in China and countries in East Asia. We treated tadpoles with 0.1, 1, 10 and 100 mu g/L AZ for 85 days and examined related parameters. The results showed that the mortality of the toads in the treatment group increased dramatically in a U-shaped dose-response relationship. The hindlimb extension and metamorphosis rate of the toads were significantly inhibited by AZ at 10 and 100 mu g/L. Under the same condition, there were significant progressive changes in the testicular structures. Moreover, we found that AZ has no significant effects on growth, sex ratios, gonadal morphology, forelimb emergence and histology in the ovaries. Our results support the idea that environmental contaminants including AZ may be relevant to global amphibian decline.