标题：Molecular implication of ADAM-15 and 17 in intrauterine adhesions
作者机构：[Liu, D] Department of Gynecology, And Obstetrics, Qilu Hospital Affiliated with Shandong University, Jinan, Shandong 250012, China, Key Laboratory of 更多
通讯作者地址：[Liu, PS]Shandong Univ, Qilu Hosp, Dept Gynecol & Obstet, Jinan 250012, Shandong, Peoples R China.
来源：European Journal of Obstetrics, Gynecology and Reproductive Biology: An International Journal
关键词：ADAM-15;ADAM-17;Intrauterine adhesions;Remodeling of uterus
摘要：Objectives To investigate the regulation of the proteins ADAM-15 and ADAM-17 in intrauterine adhesions (IUA). Study design 68 patients were found to have IUA in a study performed at our Department of Gynecology, and 18 control volunteer participants were recruited in the study. The patients with IUA were assigned to three groups according to the classification of March et al.: IUA-I (n = 28), IUA-II (n = 22), and IUA-III (n = 18). All the volunteers were assigned to the control group (Con, n = 18). The expression of ADAM-15 and ADAM-17 in the adhesive band tissue in patients and the endometrium in volunteers was detected by western blot, real-time PCR, and immunohistochemistry. Results The expression of ADAM-15 and ADAM-17 was significantly upregulated in both protein level and transcript level in IUA patients compared to that in controls. ADAM-15 expression was significantly higher in IUA-III (4.59 ± 0.15) compared to IUA-II (3.18 ± 0.12) and IUA-I (2.11 ± 0.17; P < 0.01). ADAM-17 expression was also significantly higher in IUA-III (3.25 ± 0.11) compared to IUA-II (2.21 ± 0.15) and IUA-I (1.78 ± 0.21; P < 0.01). The transcript levels of ADAM-15 and ADAM-17 showed similar patterns, and were markedly higher in grade III IUA patients compared to grade II and grade I. The severity of IUA was positively correlated to the protein and transcript expression level of ADAM-15 and ADAM-17 in uterine tissue. Conclusions The development of IUA is associated with regulation of ADAM15 and ADAM-17, which may be potential biological markers for evaluating the severity of intrauterine adhesions.