标题：The non-enzymatic glycation of LDL proteins results in biochemical alterations - A correlation study of Apo B-100-AGE with obesity and rheumatoid arthritis
作者：Li, Yi; Khan, Mohd. Shahnawaz; Akhter, Firoz; Husain, Fohad Mabood; Ahmad, Saheem; Chen, Lihui
作者机构：[Li, Yi] Shandong Univ, Dept Joint Surg, Shandong Prov Hosp, Jinan 250021, Shandong, Peoples R China.; [Khan, Mohd. Shahnawaz] King Saud Univ, Dept 更多
通讯作者：Ahmad, S;Chen, LH
通讯作者地址：[Ahmad, S]Integral Univ, Lab Glycat Biol & Metab Disorders, IIRC 1, Lucknow 226026, Uttar Pradesh, India;[Chen, LH]Shandong Univ, Shandong Prov Hosp, 更多
来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
关键词：Apo B100-AGE; Glycation; ELISA
摘要：Advanced glycation end-products (AGEs) can aggregate amid incessant inflammation, as may be available in patients with rheumatoid arthritis. D-Ribose reacts more promptly than glucose monosaccharide to the proteins and forms heterogeneous group of products known as AGEs. Obesity includes persons with provocative joint inflammation with increased lipid profile. Immunogenic evidences recommend a cross-sectional relationship between glycated LDL-Apo B100 and inflammation.; The point of this examination was to look at the connection between D-ribose glycated ApoB100 (ApoB100-AGE) with obesity and rheumatoid arthritis. The binding specificity of auto-antibodies against ApoB100-AGE antigen present in obesity and rheumatoid arthritis patient's serum were inspected by direct binding and was further established by competitive inhibition ELISA In the present study, hydroxyl radical, superoxide radical, ketoamine moieties, hydroxyl-methyl furfural (HMF) and carbonyl substances were evaluated in the patients' serum via respective specific methods. The prevalence of auto-antibodies against ApoB100-AGE antigen was recorded to be 58% and 52.86% from obese and rheumatoid arthritis patient respectively in contrast to its native analogue (P < 0.001). Moreover, the autoantibodies present in obese and arthritis patients were found to be highly specific towards ApoB100-AGE as confirmed by inhibition ELISA. (C) 2018 Published by Elsevier B.V.