标题：Rhodosalinus halophilus sp. nov., isolated from a marine solar saltern, and emended description of the genus Rhodosalinus
作者：Liu, Zhen-Wei; Wang, Tao-Jie; Yang, Hao; Lu, De-Chen; Du, Zong-Jun
作者机构：[Liu, Zhen-Wei; Wang, Tao-Jie; Yang, Hao; Lu, De-Chen; Du, Zong-Jun] Shandong Univ, Marine Coll, Weihai 264209, Peoples R China.; [Du, Zong-Jun] Sha 更多
通讯作者：Du, ZJ;Du, ZJ
通讯作者地址：[Du, ZJ]Shandong Univ, Marine Coll, Weihai 264209, Peoples R China;[Du, ZJ]Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, P 更多
来源：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
关键词：Rhodosalinus; 16S rRNA gene; phylogenetic analysis
摘要：A novel Gram-stain-negative, rod-shaped, beige-coloured, motile, facultatively anaerobic bacterium, designated as E84(T), was isolated from sediment sampled from a marine solar saltern in Wendeng, PR China. Phylogenetic analysis based on 16S rRNA gene sequencing showed that Rhodosalinus sediminis WDN1C137(T) was the closest phylogenetic relationship, with 16S rRNA gene sequence similarity of 96.9 %. Optimal growth occurred at 33-37 degrees C (range, 20-40 degrees C), at pH 7.5 (pH 7.0-8.5) and with 6.0% (w/v) NaCl (0.5-20.0 %). The sole respiratory quinone was Q-10. The major fatty acids were summed feature 8 (C-18 : 1 omega 7c and/or C-18 : 1 omega 6c), C18 : 0 and cyclo C-19 : 0 omega 8c. The polar lipids were phosphatidylglycerol, one unidentified glycolipid, two unidentified phospholipids and two unidentified lipids. The genomic DNA G+C content of strain E84(T) was 69.8 mol%. Based on the results of physiological, genotypic, phylogenetic and chemotaxonomic analyses, we concluded that strain E84(T) represents a novel species of the genus Rhodosalinus, for which the name Rhodosalinus halophilus sp. nov. is proposed. The type strain is E84(T) (=KCTC 52697(T)=MCCC 1H00231(T)).