标题:Copper ion-assisted gold nanoparticle aggregates for electrochemical signal amplification of lipopolysaccharide sensing
作者:Wang, Nan; Dai, Hongxiu; Sai, Lintao; Ma, Houyi; Lin, Meng
作者机构:[Wang, Nan; Dai, Hongxiu; Ma, Houyi; Lin, Meng] Shandong Univ, Sch Chem & Chem Engn, Key Lab Colloid & Interface Chem, State Educ Minist, Jinan 250100 更多
通讯作者:Ma, Houyi;Ma, HY;Lin, M
通讯作者地址:[Ma, HY; Lin, M]Shandong Univ, Sch Chem & Chem Engn, Key Lab Colloid & Interface Chem, State Educ Minist, Jinan 250100, Peoples R China.
来源:BIOSENSORS & BIOELECTRONICS
出版年:2019
卷:126
页码:529-534
DOI:10.1016/j.bios.2018.11.021
关键词:Lipopolysaccharide; Aptasensor; Gold nanoparticles; Copper ions; Signal; amplification
摘要:A signal amplification electrochemical aptasensor for ultrasensitive detection of lipopolysaccharide (LPS) was fabricated. The sensor was constructed with a probe of LPS aptamer and a copper ions-mediated gold nano particles aggregate (Cu/Au NA) as a signal amplification material. The Cu/Au NAs comprising copper ions (Cu2+) and L-cysteine modified AuNPs were fabricated by a self-assembly process. For functionalization of the electrode, the carboxylic group of a mercaptoacetic acid self-assembly layer was covalently coupled with the amine group of the aptamer. The aptamer with high specificity and affinity can effectively gather the dissociative LPS firstly, and the Cu/Au NAs were captured by anionic groups of the carbohydrate portions from LPS molecules based on the specific interactions. With the employment of the sandwich-type biosensor, the strategy can significantly amplify the electrochemical signal for determination of trace amount of LPS. The sensing performance of the electrochemical sensor was investigated by differential pulse voltammetry (DPV) and the stripping peak currents of Cu re-oxidized to Cu2+ was used to monitor the level of LPS. The electrochemical aptasensor exhibited excellent sensitivity toward LPS with a detection limit of 0.033 pg/mL (S/N = 3). The biosensor also exhibited a high specificity toward LPS in the presence of other common interfering substances and was easily regenerated. Furthermore, the fabricated biosensor showed a good practical application for LPS determination in human serum samples.
收录类别:EI;SCOPUS;SCIE
Scopus被引频次:1
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85056854432&doi=10.1016%2fj.bios.2018.11.021&partnerID=40&md5=5eead6e34834799c0f33a2d4f11b9ce5
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