标题:Anti-inflammatory Effect of Acetylpuerarin on Eicosanoid Signaling Pathway in Primary Rat Astrocytes
作者:Xiang, Yanxiao; Wei, Xinbing; Chen, Lin; Liu, Huiqing; Liu, Xiaoqian; Wang, Tian; Zhang, Xiumei
作者机构:[Xiang, Yanxiao; Wei, Xinbing; Chen, Lin; Liu, Huiqing; Liu, Xiaoqian; Zhang, Xiumei] Shandong Univ, Sch Med, Dept Pharmacol, Jinan 250012, Shandong, 更多
通讯作者:Zhang, XM
通讯作者地址:[Zhang, XM]Shandong Univ, Sch Med, Dept Pharmacol, 44 Wenhua Xi Rd, Jinan 250012, Shandong, Peoples R China.
来源:JOURNAL OF MOLECULAR NEUROSCIENCE
出版年:2014
卷:52
期:4
页码:577-585
DOI:10.1007/s12031-013-0113-6
关键词:Acetylpuerarin; Inflammation; Rat astrocytes in primary culture;; Eicosanoid; GroupV sPLA(2); COX and LOX
摘要:Astrocytes activation has been implicated in the inflammatory responses underlying brain injury and neurodegenerative diseases including bacterial infections, cerebral ischemia, and Parkinson's diseases. Acetylpuerarin is a newly modified isoflavone based on puerarin that has neuroprotective and antioxidant effects. In this study, we investigated the anti-inflammatory action of acetylpuerarin in regulating the eicosanoids generation and its underlying molecular mechanisms in lipopolysaccharide (LPS)-induced production of arachidonic acid (AA) metabolites in primary rat astrocytes. The results showed that acetylpuerarin concentration dependently inhibited the LPS-induced production of AA metabolites such as prostaglandin E-2 (PGE(2)) and leukotriene C-4 (LTC4), and acetylpuerarin significantly attenuated the expression and immunoreactivity of group V secretory phospholipase A(2) (sPLA(2)) protein induced by LPS in astrocytes. Furthermore, in astrocytes pretreated with acetylpuerarin, the time course of phosphorylation of extracellular signal-regulated kinase (ERK)1/2 and of cytosolic PLA(2) alpha (cPLA(2)alpha) and expression of transcription factors, nuclear factor kappa B (NF-kappa B), was markedly truncated. Acetylpuerarin concentration dependently abolished the LPS-induced expressions of AA-metabolizing enzymes including cyclooxygenase-2 (COX-2) and lipooxygenase-5 (LOX-5). This study indicates that acetylpuerarin inhibited LPS-induced AA-metabolizing enzymes and AA metabolites in astrocytes via downregulation expression of group V sPLA(2) and phosphorylation of ERK1/2, cPLA(2)alpha, and NF-kappa B. These findings reveal, in part, the molecular basis underlying the anti-inflammatory properties of acetylpuerarin.
收录类别:SCIE
WOS核心被引频次:8
资源类型:期刊论文
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