标题：Response of Bacteria and Fungi in Soil Microcosm under the Presence of Pesticide Endosulfan
作者：Zhang, Jian; Qin, Jing; Zhao, Congcong; Liu, Cui; Xie, Huijun; Liang, Shuang
作者机构：[Zhang, Jian; Zhao, Congcong; Liang, Shuang] Shandong Univ, Sch Environm Sci & Engn, Jinan 250100, Peoples R China.; [Qin, Jing; Xie, Huijun] Shando 更多
通讯作者地址：[Xie, HJ]Shandong Univ, Environm Res Inst, Jinan 250100, Peoples R China.
来源：WATER AIR AND SOIL POLLUTION
关键词：Endosulfan; Bacteria; Fungi; qPCR; DGGE
摘要：Because of its long half-life, endosulfan can persist for a long time in the environment, especially in soil. However, little is known about its effect on fungi, which is an important part of microorganisms in soil. In this study, agricultural soil treated with endosulfan (0.1 and 1.0 mg kg(-1)) in a laboratory experiment was analyzed over 42 days. The effect of endosulfan on bacterial and fungal quantity and community structure were determined by quantitative polymerase chain reaction (qPCR) and denaturing gradient gel electrophoresis (DGGE). The results revealed that endosulfan was removed more than 50 % after 42 days, and its removal fitted single first-order kinetics. The exposure to endosulfan caused a short-lived inhibition on fungal and bacterial quantity, but no effect was observed in both treatments after 42 days. Furthermore, this inhibition was greater in higher endosulfan-treated soil. A significant change in bacterial community structure was found in both treatments after endosulfan application, while the change of fungal community structure was observed only in 1 mg kg(-1) endosulfan treatment.