标题：The Podocyte Count Detected by an Improved Immunocytochemical Method has Higher Diagnostic Efficiency than Enzyme-Linked Immunosorbent Assay and Serum Cystatin C to Evaluate the Early Stage Damage of Glomerular
作者：Li, Li; Wang, Jing; Li, Yi; Lu, Chao; Liu, Yiqing; Zhang, Zhifen; Wang, Zi-e; Zhang, Jian; Lu, Zhiming
作者机构：[Li, Li; Lu, Chao; Liu, Yiqing; Zhang, Zhifen; Wang, Zi-e; Zhang, Jian; Lu, Zhiming] Shandong Univ, Dept Lab Med, Shandong Prov Hosp, 324 Jingwu Rd, J 更多
通讯作者：Zhang, J;Lu, ZM
通讯作者地址：[Zhang, J; Lu, ZM]Shandong Univ, Dept Lab Med, Shandong Prov Hosp, 324 Jingwu Rd, Jinan 250021, Shandong, Peoples R China.
来源：JOURNAL OF CLINICAL LABORATORY ANALYSIS
关键词：cystatin C; diagnostic efficiency; enzyme-linked immunosorbent assay;; glomerular damage; immuocytochemical stain; podocyte count
摘要：BackgroundIn renal diseases, earlier injury to glomerular may lead to the abscission of podocyte. The number of podocyte in urine may reflect the severity of glomerular damage. Podocalyxin (PCX) was considered as a podocyte marker. Many methods were used to detect podocyte. Applications of these methods were limited by tricky, expensive, and low accuracy. Here, we improved an immunocytochemical method to count the number of podocyte in urine.; MethodsIn this study, we counted the numbers of podocyte in urine by our improved method and detected the PCX levels in urine by enzyme-linked immunosorbent assay (ELISA) in glomerulopathy patients and healthy controls. The serum levels of cystatin C (CysC), blood urea nitrogen (BUN), creatinine (CR), uric acid (URIC), and 2-Microglobulin (2-MG) in all subjects were detected. Correlation analysis and diagnostic efficiencies comparisons among immuocytochemical method, ELISA, and the biochemistry index were also performed.; ResultsThe podocyte counts in patients were significantly higher than controls. Podocytes counts positively correlated with PCX concentrations and the serum CysC. The podocyte count had higher diagnostic efficiency than PCX concentrations detected by ELISA and serum CysC.; ConclusionsThe podocyte count detected by our improved method had higher diagnostic efficiency than ELISA and serum CysC.