标题:Engineering Pseudomonas protegens Pf-5 to improve its antifungal activity and nitrogen fixation
作者:Jing X.; Cui Q.; Li X.; Yin J.; Ravichandran V.; Pan D.; Fu J.; Tu Q.;等 更多
作者机构:[Jing, X] State Key Laboratory of Microbial Technology, School of Life Science, Shandong University-Helmholtz Institute of Biotechnology, Shandong Uni 更多
通讯作者:Wang, H(wanghailong@sdu.edu.cn)
通讯作者地址:[Wang, H] State Key Laboratory of Microbial Technology, School of Life Science, Shandong University-Helmholtz Institute of Biotechnology, Shandong Uni 更多
来源:Microbial Biotechnology
出版年:2018
DOI:10.1111/1751-7915.13335
摘要:In agricultural production, sustainability is currently one of the most significant concerns. The genetic modification of plant growth-promoting rhizobacteria may provide a novel way to use natural bacteria as microbial inoculants. In this study, the root-colonizing strain Pseudomonas protegens Pf-5 was genetically modified to act as a biocontrol agent and biofertilizer with biological nitrogen fixation activity. Genetic inactivation of retS enhanced the production of 2,4-diacetylphloroglucinol, which contributed for the enhanced antifungal activity. Then, the entire nitrogenase island with native promoter from Pseudomonas stutzeri DSM4166 was introduced into a retS mutant strain for expression. Root colonization patterns assessed via confocal laser scanning microscopy confirmed that GFP-tagged bacterial were mainly located on root surfaces and at the junctions between epidermal root cells. Moreover, under pathogen and N-limited double treatment conditions, the fresh weights of seedlings inoculated with the recombinant retS mutant-nif strain were increased compared with those of the control. In conclusion, this study has innovatively developed an eco-friendly alternative to the agrochemicals that will benefit global plant production significantly. © 2018 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.
收录类别:SCOPUS
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85056753405&doi=10.1111%2f1751-7915.13335&partnerID=40&md5=7f8e93fc42aa3c6ab5d073d783100b01
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