标题：Synthesis, characterization, and properties of novel hydrophobically associating fluorinated copolymers for DNA delivery
作者：Wei Kuang;Xiaodong Zhao
作者机构：[Kuang, W] School of Chemistry and Pharmaceutical Engineering, Shandong Polytechnic University, Jinan 250353, China, School of Chemistry and Chemical 更多
通讯作者地址：[Kuang, W]Shandong Polytech Univ, Sch Chem & Pharmaceut Engn, Jinan 250353, Peoples R China.
来源：Reactive & functional polymers
关键词：hydrophobic association;block copolymer;fluorocarbon;gene vector
摘要：Novel fluorinated associative copolymers poly(ethylene glycol)-b-poly(2-(perfluorohexyl)ethyl methac-rylate (PFHEMA)-co-3-[N-(2-methacroyloylethyl)-N.N-dimethylammonio]-propane sulfonate (DMAPS)) (PEG-b-PPFHEMA/PDMAPS) were prepared as a type of non-viral gene vector. The series of PEG-b-PFHE-MA/DMAPS with different molecular weights and compositions were characterized by gel permeation chromatography-multi-angle light scattering, ~1H NMR, ~(19)F NMR, and elemental analysis. The interactions of the copolymers with calf thymus DNA and the morphologies of the resulting complexes were studied by fluorescence spectroscopy, dynamic light scattering, and transmission electron microscopy. In addition, the DNA-polymer interaction, as determined by the gel retardation assay, and the low cytotoxicity of PEG-b-PPFHEMA/PDMAPS demonstrated that these copolymers have good DNA binding capacity and potential for high performance in the practical application of DNA delivery.