标题： LncRNA C2dat1 Promotes Cell Proliferation, Migration, and Invasion by Targeting MiR-34a-5p in Osteosarcoma Cells.
作者： Jia, Daofu; Niu, Yanping; Li, Dongling; Liu, Zhaorui
作者机构：[ Jia, Daofu; Jia D] Department of Emergency Medicine, Jinan Central Hospital, Jinan 250013, Shandong, China.;[ Niu, Yanping; Niu Y] Department of Out 更多
来源： Oncology research
摘要： Osteosarcoma is a highly aggressive malignant bone tumor with poor prognosis in cases. Evidence suggested that lncRNAsare deregulated in multiple cancers. In this study, we investigated the role of the lncRNA C2dat1 on the biological functions of osteosarcoma cells. The expressions of C2dat1, miR-34a-5p and Sirt1 in human osteosarcoma cells were altered by transfection with their specific vectors/shRNA, or mimic/inhibitor. Then, cell viability, migration, invasion and apoptosis were respectively assessed post-transfection. The mRNA and protein levels of C2dat1, miR-34a-5p and Sirt1 were detected by qRT-PCR and western blot. The results showed that C2dat1 suppression reduced cell viability, invasion and migration, while increased cell apoptosis in OS-732 cells. MiR-34a-5p was a target of C2dat1, that the expression of miR-34a-5p was downregulated when C2dat1 overexpression, while was negatively regulated the Sirt1 expression. MiR-34a-5p overexpression inhibited cell viability, migration, invasion and promoted cell apoptosis in osteosarcoma cells by downregulation of Sirt1. Furthermore, miR-34a-5p overexpression deactivated p38/ERK/AKT and Wnt-ss-catenin signaling pathways by inhibition of Sirt1.