标题：Protection via a ROM4 DNA vaccine and peptide against Toxoplasma gondii in BALB/c mice
作者：Han, Yali; Zhou, Aihua; Lu, Gang; Zhao, Guanghui; Wang, Lin; Guo, Jingjing; Song, Pengxia; Zhou, Jian; Zhou, Huaiyu; Cong, Hua; He 更多 作者机构：[Han, Yali; Lu, Gang; Guo, Jingjing; Song, Pengxia; Zhou, Jian; Zhou, Huaiyu; Cong, Hua; He, Shenyi] Shandong Univ, Sch Med, Dept Parasitol, Jinan 250 更多
通讯作者地址：[He, SY]Shandong Univ, Sch Med, Dept Parasitol, Jinan 250012, Shandong, Peoples R China.
来源：BMC INFECTIOUS DISEASES
关键词：Toxoplasma gondii; Vaccine; ROM4; Peptide; Bioinformatics; Immunization; strategy
摘要：Background: Toxoplasma gondii (T. gondii) is an obligate intracellular protozoan parasite with a broad host range including most warm-blooded animals, including humans. T. gondii surface antigen 1 (SAG1) is a well-characterized T. gondii antigen. T. gondii expresses five nonmitochondrial rhomboid intramembrane proteases, TgROM1-5. TgROM4 is uniformly distributed on the surface of T. gondii and involved in regulating MIC2, MIC3, MIC6, and AMA1 during T. gondii invasion of host cells. Bioinformatics have predicted ROM4 B-cell and T-cell epitopes. Immunization strategy is also a key factor in determining the effectiveness of the immune response and has gained increasing attention in T. gondii vaccine research. In this study, we used a DNA prime-peptide boost vaccination regimen to assess the protective efficacy of various vaccination strategies using TgROM4.; Methods: We identified a polypeptide (YALLGALIPYCVEYWKSIPR) using a bioinformatics approach, and immunized mice using a DNA-prime and polypeptide-boost regimen. BALB/c mice were randomly divided into six groups, including three experimental groups (peptide, pROM4 and pROM4/peptide) and three control groups (PBS, pEGFPC1 and pSAG1). Mice were then immunized intramuscularly four times. After immunization, IgG and cytokine productions were determined using enzyme-linked immunosorbent assays. The survival time of mice was evaluated after challenge with tachyzoites of T. gondii RH strain. Additionally, the number of cysts in the brain was determined after intragastric challenge with cysts of T. gondii PRU strain.; Results: Mice vaccinated with different immunization regimens (peptide, pROM4 and pROM4/peptide) elicited specific humoral and cellular responses, with high levels of IgG, IgG2a, and interferon (IFN)-gamma. Moreover, IgG, IgG2a and IFN-gamma levels were highest in the pROM4/peptide group. Immunized mice, especially those in the pROM4/peptide group, had prolonged survival times after challenge with tachyzoites and reduced numbers of brain cysts after infection compared with negative controls.; Conclusion: A DNA prime-peptide boost regimen based on ROM4 elicited the highest level of humoral and cellular immune responses among immunization regimens, and may be a promising approach to increase the efficacy of DNA immunization.