标题：Novel pyrazoline-based fluorescent probe for detecting thiols and its application in cells
作者：Zhang, Rong-Rong; Zhang, Jin-Feng; Wang, Sheng-Qing; Cheng, Yan-Long; Miao, Jun-Ying; Zhao, Bao-Xiang
作者机构：[Zhang, Rong-Rong; Wang, Sheng-Qing; Cheng, Yan-Long; Zhao, Bao-Xiang] Shandong Univ, Sch Chem & Chem Engn, Inst Organ Chem, Jinan 250100, Peoples R C 更多
通讯作者地址：[Miao, JY]Shandong Univ, Sch Life Sci, Inst Dev Biol, Jinan 250100, Peoples R China.
来源：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
关键词：Pyrazoline; Fluorescent probe; Selective; Thiol
摘要：A new compound, N-(4-(1,5-diphenyl-4,5-dihydro-1H-pyrazol-3-yl)phenyl)-acrylamide (probe L), was designed and synthesized as a highly sensitive and selective fluorescent probe for recognizing and detecting thiol from other amino acids. On being mixed with thiol in buffered DMSO:HEPES = 1:1 solution at pH 7.4, the probe exhibited the blue emission at 474 nm. This probe is very sensitive and displayed a linear fluorescence off-on response to thiol. The fluorescence emission of the probe is pH independent in the physiological pH range. Living cell imaging of HeLa cells confirmed its cell permeability and its ability to selectively detect thiol in cells. The structure of the probe was characterized by IR, NMR and HRMS spectroscopy analysis. (C) 2014 Elsevier B.V. All rights reserved.