标题:Biosynthesis of magnetic nanostructures in a foreign organism by transfer of bacterial magnetosome gene clusters
作者:Isabel Kolinko;Anna Loh?e;Sarah Borg;Oliver Raschdorf;Christian Jogler;Qiang Tu;Mihály Pósfai;éva Tompa;Jürgen M. Plitzko;Andrea
作者机构:[Kolinko, I] Ludwig-Maximilians-Universität München, Department of Biology I, Großhaderner Straße 2-4, 82152 Martinsried, Germany;[ Lohße, A] Ludwig-M 更多
通讯作者:Zhang, YM
通讯作者地址:[Zhang, YM]Shandong Univ, Helmholtz Joint Inst Biotechnol, State Key Lab Microbial Technol, Life Sci Coll, Jinan 250100, Peoples R China.
来源:Nature nanotechnology
出版年:2014
卷:9
期:3
页码:193-197
DOI:10.1038/NNANO.2014.13
关键词:Biosynthesis;magnetic nanostructures;bacterial magnetosome gene clusters
摘要:The synthetic production of monodisperse single magnetic domain nanoparticles at ambient temperature is challenging~(1,2). In nature, magnetosomes-membrane-bound magnetic nanocrystals with unprecedented magnetic properties-can be biomineralized by magnetotactic bacteria~3. However, these microbes are difficult to handle. Expression of the underlying biosynthetic pathway from these fastidious microorganisms within other organisms could therefore greatly expand their nanotechnological and biomedical applications~(4,5). So far, this has been hindered by the structural and genetic complexity of the magnetosome organelle and insufficient knowledge of the biosynthetic functions involved. Here, we show that the ability to biomineralize highly ordered magnetic nanostructures can be transferred to a foreign recipient. Expression of a minimal set of genes from the magnetotactic bacterium Magnetospirillum gryphiswaldense resulted in magnetosome biosynthesis within the photosynthetic model organism Rhodospirillum rubrum. Our findings will enable the sustainable production of tailored magnetic nanostructures in biotechnologically relevant hosts and represent a step towards the endogenous magnetization of various organisms by synthetic biology.
收录类别:SCOPUS;SCIE
WOS核心被引频次:61
Scopus被引频次:67
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84895926490&doi=10.1038%2fnnano.2014.13&partnerID=40&md5=9137496571df1ebfb5bb5c2f914bba6c
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