标题：HMBOX1 negatively regulates NK cell functions by suppressing the NKG2D/DAP10 signaling pathway
作者：Wu, Longyan; Zhang, Cai; Zhang, Jian
作者机构：[Wu Longyan] Institute of Immunopharmacology and Immunotherapy, School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong 250012, China. 更多
通讯作者地址：[Zhang, J]Shandong Univ, Sch Pharmaceut Sci, Inst Immunopharmacol & Immunotherapy, 44 Wenhua W Rd, Jinan 250012, Peoples R China.
关键词：HMBOX1; NK cells; NKG2D
摘要：HMBOX1 is a new member of the homeobox family. Homeobox members have been reported to participate in embryonic development and systemic metabolism, but the function of HMBOX1 remains unclear, especially in the hematopoietic system. Here, we show that HMBOX1 is expressed at a high level in primary human NK cells but is expressed at much lower levels in NK cell lines. Overexpression of HMBOX1 significantly inhibited NK cell activities, including natural cytotoxicity against tumor cells, the level of CD107a (a marker protein for degranulation) and the production of cytolytic proteins (perforin and granzymes). More interestingly, HMBOX1 negatively regulated the expression of NKG2D and the activation of the NKG2D/DAP10 signaling pathway in NK cells. This effect was reversed by knocking down HMBOX1. Taken together, these findings demonstrate that HMBOX1 may act as a negative regulator of NK cell functions via suppressing the NKG2D/DAP10 signaling pathway. Cellular & Molecular Immunology (2011) 8, 433-440; doi:10.1038/cmi.2011.20; published online 27 June 2011