标题：One-step simple isolation method to obtain both epidermal and dermal stem cells from human skin specimen
作者：Qian H.; Leng X.; Wen J.; Zhou Q.; Xu X.; Wu X.
作者机构：[Qian, H] Laboratory for Tissue Engineering and Regeneration, and Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatolog 更多
通讯作者地址：[Wu, X] Laboratory for Tissue Engineering and Regeneration, and Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, 更多
来源：Methods in Molecular Biology
关键词：Adult; Culture; Dermal fibroblasts; Epidermal stem cells; Human; Y-27632
摘要：Stem cells play a crucial role in maintaining and repairing tissues during homeostasis and following injury. The efficient procurement of high quantity and quality of skin stem cells is important for both laboratory studies and clinical applications. Here, we describe a one-step isolation procedure to efficiently obtain both epidermal and dermal cell population from human skin specimen, based on the different influence of the Rho kinase inhibitor Y27632 on the growth of epidermal and dermal cells during the initial culture. Compared with the conventional methods, our protocol shows that it is simpler and less time consuming and can efficiently obtain the high quality of skin stem cells and can maintain the stem cell features after culture expansion. © Springer Science+Business Media New York 2018.