标题：Determination of salbutamol in human plasma and urine using liquid chromatography coupled to tandem mass spectrometry and its pharmacokinetic study
作者机构：Institute of Clinical Pharmacology, Qilu Hospital of Shandong University, 107 West Wenhua Road;Institute of Clinical Pharmacolog
通讯作者地址：[Guo, RC]Shandong Univ, Inst Clin Pharmacol, Qilu Hosp, 107 W Wenhua Rd, Jinan 250012, Peoples R China.
来源：Biomedical Chromatography: An International Journal Devoted to Research in Chromatographic Methodologies and Their Applications in the Biosciences
摘要：A sensitive and selective liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was developed and validated for the determination of salbutamol in human plasma and urine, and successfully applied to the pharmacokinetic study of salbutamol in Chinese healthy volunteers after inhalation of salbutamol sulfate aerosol. Salbutamol and the internal standard (IS) acetaminophen in plasma and urine were extracted with ethyl acetate, separated on a C 18 reversed-phase column, eluted with mobile phase of acetonitrile-ammonium acetate (5m m; 30:70, v/v), ionized by positive ion pneumatically assisted electrospray and detected in the multi-reaction monitoring mode using precursor→product ions of m/z 240.2→148.1 for salbutamol and 152→110 for the IS. The lower limits of quantitation of salbutamol in human plasma and urine by this method were 0.02 and 1ng/mL, respectively. The specificity, matrix effect, recovery, sensitivity, linearity, accuracy, precision and several stabilities were validated for salbutamol in human plasma and urine. In conclusion, the validation results showed that this method is robust, specific and sensitive, and can successfully fulfill the requirement of clinical pharmacokinetic study of salbutamol in healthy Chinese volunteers.