标题：Study of a humanized inhibitory anti-platelet glycoprotein VI phage antibody from a phage antibody library
作者：Liu, Qinghong; Zhang, Chunmei; Yu, Lingjia; Shi, Yongyu; Zhang, Liping; Peng, Jun; Ji, Xuebin; Hou, Ming
作者机构：[Liu, Qinghong; Yu, Lingjia; Peng, Jun; Ji, Xuebin; Hou, Ming] Shandong Univ, Qilu Hosp, Dept Hematol, 107 West Wenhua Rd, Jinan 250012, Shandong, Peo 更多
通讯作者：Ji, XB;Hou, M
通讯作者地址：[Ji, XB; Hou, M]Shandong Univ, Qilu Hosp, Dept Hematol, 107 West Wenhua Rd, Jinan 250012, Shandong, Peoples R China.
关键词：Anti-GPVI auto-antibody; Platelet aggregation; Phage surface display; technology; Humanized single chain Fv; Immune thrombocytopenia
摘要：Objective: The aims of the study were to study the effect of anti-platelet glycoprotein (GP) VI auto-antibodies on platelet aggregation and use phage surface display technology to produce anti-platelet GPVI phage antibody fragment, which may be developed to inhibit platelet aggregation in the treatment of cardiovascular disease.; Methods: Plasma samples from patients with immune thrombocytopenia (ITP) were screened by monoclonal antibody immobilization of the platelet antigen assay and the platelet aggregation test for anti-platelet GPVI auto-antibody with an inhibitory effect. The humanized anti-platelet GPVI phage antibody was produced by phage surface display technology. The function of the phage antibody fragment against platelet aggregation was examined by the platelet aggregation test.; Results: Of 726 ITP patients, 2 (0.27%) patients' plasma significantly inhibited platelet aggregation induced by collagen-1. After five rounds of selection, enrichment, and purification, a soluble phage antibody fragment was produced, which can inhibit platelet aggregation induced by collagen-1. The results demonstrate that only a few of the screened anti-platelet GPVI auto-antibodies showed an inhibitory effect on platelet aggregation.; Discussion: A completely humanized anti-GPVI soluble phage antibody can be produced by phage surface display technology. The antibody was able to specifically block collagen-induced platelet aggregation without influencing the aggregation responses to other agonists.; Conclusions: Results of the present study suggest that very few anti-platelet GPVI auto-antibodies inhibit the aggregation function of platelet. The humanized anti-platelet GPVI produced by phage surface display technology is promising to be used to inhibit platelet aggregation in the treatment of cardiovascular disease.