标题：A portable membrane-based analysis system for rapid and sensitive detection of Bacteria
作者：Liu, Jian ;Shang, Hui ;Wang, Xinpei ;Liu, Changchun
作者机构：[Liu, Jian ;Wang, Xinpei ;Liu, Changchun ] School of Control Science and Engineering, Shandong University, Jinan, China;[Shang, Hui ] Shandong Provinc 更多
会议名称：2010 8th World Congress on Intelligent Control and Automation, WCICA 2010
会议日期：7 July 2010 through 9 July 2010
来源：Proceedings of the World Congress on Intelligent Control and Automation (WCICA)
关键词：Bacteria; Fluorescent probe; Fluoroimmunoassay; Membrane-based
摘要：In this paper, we report a membrane filter-based detection system of bacteria using streptavidin functionalized quantum dots (SA-QDs) as a fluorescent probe. The aimed E. coli cells were marked by quantum dots through immuno-recognition, and then the marked bacteria were enriched by a low-fluorescence optical aluminium oxide membrane. A portable system was prepared for measurement of fluorescence on the membrane, which was correlation with the quantity of the marked bacteria. The whole testing time including sample pretreatment could be completed within 30 minutes. Comparing with the traditional plate count method, bacteria could be detected specifically and rapidly without time consuming cultivation. Our results showed that the output of instrument had a good correlation with total bacteria count in the range of 102 ∼ 104 CFU/mL. The advantages of simple and efficient testing make this method potential attractive for in site detection of bacteria. © 2010 IEEE.