标题：TLR7 regulates dendritic cell-dependent B-cell responses through BlyS in immune thrombocytopenic purpura
作者：Yu, Huiming; Liu, Yunfang; Han, Junqing; Yang, Zhe; Sheng, Wei; Dai, Honghai; Wang, Yingjie; Xia, Tingyi; Hou, Ming
作者机构：[Yu, Huiming; Han, Junqing; Yang, Zhe; Sheng, Wei; Dai, Honghai] Shandong Univ, Prov Hosp, Ctr Canc, Jinan 250012, Shandong, Peoples R China.; [Yu, 更多
通讯作者地址：[Han, JQ]Shandong Univ, Prov Hosp, Ctr Canc, 44 Wen Huaxi Rd, Jinan 250012, Shandong, Peoples R China.
来源：EUROPEAN JOURNAL OF HAEMATOLOGY
关键词：toll-like receptor 7; B-lymphocyte stimulator; immune thrombocytopenic; purpura; B cells; dendritic cells
摘要：Objectives:; Immune thrombocytopenic purpura (ITP) is an autoimmune disorder in which anti-platelet antibodies induce platelet destruction owing to an imbalanced immune response. Several studies have established that B cells play an important role in the production of anti-platelet antibodies and that dendritic cells (DC) are professional antigen-presenting cells of the immune system that may lead to the development of autoantibody through B cells. We aimed at investigating the role of B cells and DC in the pathogenesis of ITP through B-lymphocyte stimulator (BlyS) and toll-like receptor 7 (TLR7) signals.; Methods:; Twenty-two patients with ITP and 20 healthy controls were enrolled in this study. Serum BlyS, its mRNA and TLR7 mRNA were measured using ELISA kits or RT-PCR, and CD14+ or CD19+ monocytes were investigated for the pathogenesis of ITP with in vitro culture systems.; Results:; We demonstrated that serum BlyS levels in patients with ITP were significantly higher than those in healthy controls and that there was a positive correlation between serum BlyS levels and glycoprotein-specific antibody levels in patients with ITP. We found that TLR7 regulates dendritic cell-dependent B-cell responses through BlyS in patients with ITP. Dendritic cells stimulated with R848 (TLR7 ligand) are able to produce vast amounts of BlyS, which is crucial for B-cell survival, proliferation and differentiation, and increase anti-platelet antibodies production in in vitro coculture systems.; Conclusions:; These findings provide new insights into the pathogenesis of ITP by which TLR7 regulates DC-dependent B-cell responses through BlyS.