标题：NF-B signaling maintains the survival of cadmium-exposed human renal glomerular endothelial cells
作者：Zhang, Hongyan; Li, Liqun; Wang, Yifan; Dong, Fengyun; Chen, Xiaocui; Liu, Fuhong; Xu, Dongmei; Yi, Fan; Kapron, Carolyn M.; Liu, Ju
作者机构：[Zhang, Hongyan] Taishan Med Univ, Xintai Hosp, Xintai City Peoples Hosp, Dept Cardiovasc Med, Xintai 271200, Shandong, Peoples R China.; [Li, Liqun 更多
通讯作者地址：[Liu, J]Shandong Univ, Shandong Prov Qianfoshan Hosp, Med Res Ctr, Lab Microvasc Med, 16766 Jingshi Rd, Jinan 250014, Shandong, Peoples R China.
来源：INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
关键词：cadmium; nuclear factor-B signaling; renal glomerular endothelial cells;; c-Jun N-terminal kinase signaling; apoptosis
摘要：The kidney is one of the primary organs targeted by cadmium (Cd), a widely distributed environmental pollutant. The glomerular endothelium is the major component of the glomerular filtration barrier. However, the effects of Cd on glomerular endothelial cells remain largely unknown. For this purpose, we aimed to determine the effects of low dose Cd on the survival of human renal glomerular endothelial cells (HRGECs). Cultured HRGECs were exposed to 4 mu M cadmium chloride (CdCl2) and examined at different time-points. We found that Cd activates the nuclear factor-B (NF-B) pathway without inducing the apoptosis of HRGECs. Pre-treating the cells with pyrrolidine dithiocarbamate (PDTC), a potent NF-B inhibitor, prior to Cd exposure triggered extensive cell death (73.5%). In addition, Cd activates the c-Jun N-terminal kinase (JNK) pathway, and inhibition of the NF-B pathway significantly elevates Cd-induced JNK phosphorylation in HRGECs (p<0.01). The combination treatment of PDTC and SP600125, a JNK pathway inhibitor, increased the survival of Cd-stimulated HRGECs compared with those cells treated with PDTC alone (p<0.05). Taken together, these findings demonstrate that the NF-B pathway plays an essential role in maintaining the survival of Cd-exposed HRGECs.