标题:Investigating the stability of gold nanorods modified with thiol molecules for biosensing
作者:Gong, Yongxiang ;Liu, Jian ;Liu, Runcong ;Wang, Jiaojiao ;Niu, Chunmei ;Zhu, Wenfeng ;Xu, Dongmei ;Hu, Zhongbo ;Li, Min ;Zhao, Yuliang
作者机构:[Gong, Yongxiang ;Liu, Jian ;Liu, Runcong ;Wang, Jiaojiao ;Zhu, Wenfeng ;Li, Min ;Zhao, Yuliang ] CAS Key Laboratory for Biomedical Effects of Nanomat 更多
通讯作者:Li, Min
来源:RSC Advances
出版年:2015
卷:6
期:1
页码:174-178
DOI:10.1039/c5ra22133a
摘要:Gold nanorods (GNRs) modified with functional molecules are useful in chemical and biological sensing. In this work, the extinction spectrum of purified GNRs prepared by seed-mediated growth is systematically characterized by UV-visible absorption spectroscopy to test its stability before and after alkane-thiol modification. The results show that purification of GNRs makes the GNRs less stable, while a GNRs colloid solution functionalized by 11-mercaptoundecanoic acid (MUA), 3,3′-dithiobis[6-nitrobenzoic acid]bis(succinimide)ester (DSNB), or 16-mercaptohexadecanoic acid (MHA), respectively is stable over time. Bovine serum albumin (BSA) as a sample protein can be successfully attached to DSNB modified GNRs and form stable BSA-DSNB-GNRs probes. The red-shift observed in the extinction spectrum due to the BSA attachment is consistent over repeated experiments. Finally, anti-PSA (prostate-specific antigen) as a capture antibody is also attached to DSNB-modified GNRs. The attached anti-PSA is capable of interacting with prostate-specific antigens and induces a further red-shift, suggesting potential applications of thiol modified GNRs in bio-sensing.
© 2016 The Royal Society of Chemistry.
收录类别:EI
资源类型:期刊论文
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