标题：Hybrid inflorescences derived from gamma-fusion of Arabidopsis thaliana with Bupleurum scorzonerifolium
作者：Wang, M.;Peng, Z.;Hong, S.;Zhi, D.;Xia, G.
作者机构：[Wang, M] School of Life Sciences, Shandong University, Shan Da Nan Lu 27, Jinan 250100, China;[ Peng, Z] Key Laboratory for Genetic Improvement of Cr 更多
通讯作者地址：[Xia, GM]Shandong Univ, Sch Life Sci, Key Lab Plant Cell Engn & Germplasm Innovat, Minist Educ, Jinan 250100, Peoples R China.
来源：Protoplasma: An International Journal of Cell Biology
关键词：Arabidopsis thaliana (L.) Heynh;Asymmetric somatic hybridization;Bupleurum scorzonerifolium Willd;Gamma ray (γ-ray) irradiation;Hybrid inflorescence and flower
摘要：In our early experiments, a variety of Bupleurum scorzonerifolium-like somatic hybrid plants were obtained from protoplast fusion between Arabidopsis thaliana and UV-treated/untreated B. scorzonerifolium. To compare the effects of UV and γ-ray irradiation on the B. scorzonerifolium partner and obtain Arabidopsis-like hybrids, we designed a novel combination of somatic hybridization between A. thaliana and B. scorzonerifolium. Before protoplast isolation and fusion, the suspension cells of B. scorzonerifolium were irradiated by gamma ray (~(60)Co, 50 Gy with 1.3 Gy min ~(-1)). Both parental protoplasts lost regeneration capacity, but over 100 somatic hybrids restored the capacity and developed to Arabidopsis-like inflorescences and flowers with some characteristics of B. scorzonerifolium. Some hybrid flowers showed yellow sepal, petal, or carpel, whose color was similar to the petal of B. scorzonerifolium; the others had silique of Arabidopsis with angularity of B. scorzonerifolium, and their parts possessed five stamens, the same as B. scorzonerifolium. Cytological analysis showed that three hybrids had Arabidopsis-like karyotypes. Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeats (SSR) profiles revealed that both parental fragments were amplified from these hybrids. These results indicated chromatin introgression from B. scorzonerifolium to A. thaliana, which may be related to the complementation of hybrid inflorescence and flower generation.