标题:A novel lipid droplets-targeting ratiometric fluorescence probe for hypochlorous acid in living cells
作者:Wu, Wen-Li; Ma, Han-Lin; Xi, Long-Long; Huang, Miao-Fei; Wang, Kai-Ming; Miao, Jun-Ying; Zhao, Bao-Xiang
作者机构:[Wu, Wen-Li; Xi, Long-Long; Huang, Miao-Fei; Zhao, Bao-Xiang] Shandong Univ, Sch Chem & Chem Engn, Inst Organ Chem, Jinan 250100, Shandong, Peoples R 更多
通讯作者:Wang, KaiMing;Zhao, BX;Wang, KM
通讯作者地址:[Zhao, BX]Shandong Univ, Sch Chem & Chem Engn, Inst Organ Chem, Jinan 250100, Shandong, Peoples R China;[Wang, KM]Univ Jinan, Sch Biol Sci & Technol, 更多
来源:TALANTA
出版年:2019
卷:194
页码:308-313
DOI:10.1016/j.talanta.2018.10.006
关键词:Ratiometric fluorescence; Lipid droplets-targeting; Endogenous; hypochlorous acid; Cell image
摘要:Lipid droplets were found to be involved in many organism activities. Here, a lipid droplets-targeted near infrared fluorescence probe (named XHZ) for ratiometric detection of endogenous hypochlorous acid/hypochlorite (HClO/ClO-) in living cells was developed, which was constructed by a coumarin moiety and a malononitrile derivative. XHZ could detect HClO/ClO- with high selectivity and sensitivity in a ratiometric manner based on FRET (Forster Resonance Energy Transfer) mechanism. The two well-resolved emission (470/672 nm) bands could ensure accurate detection of HClO/ClO- in vitro as well as in vivo. XHZ was successfully used for ratiometric fluorescence imaging of exogenous and endogenous HClO/ClO- in RAW264.7 cells. A good linear relationship between the fluorescence intensity ratios of the two emissions and HClO/ClO- concentrations from 0 to 40 mu m was obtained. Importantly, XHZ could localize mainly in lipid droplets of RAW264.7 cells. To the best of our knowledge, XHZ is the first lipid droplets-targeted ratiometric fluorescence probe for HClO/ClO-.
收录类别:EI;SCOPUS;SCIE
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85055291796&doi=10.1016%2fj.talanta.2018.10.006&partnerID=40&md5=6914becc3b875425b3b145452f15c0e2
TOP