标题：Glycoengineering of Natural Killer Cells with CD22 Ligands for Enhanced Anticancer Immunotherapy
作者：Wang X.; Lang S.; Tian Y.; Zhang J.; Yan X.; Fang Z.; Weng J.; Lu N.;等 更多 作者机构：[Wang, X] Department of Biomaterials, College of Materials, Xiamen University, Xiamen, 361005, China;[ Lang, S] Depts. of Chem. and Biomed. Engineerin 更多
通讯作者地址：[Li, Z] Xiamen Nuokangde Biological Technology Co., Ltd.China;
来源：ACS Applied Materials and Interfaces
摘要：Adoptive transfer of immune cells is being actively pursued for cancer treatment. Natural killer (NK) cells, a class of cytotoxic immune cells, generally lack inherent selectivities toward cancer. To bestow tumor-targeting abilities and enhance anticancer efficacy, a new strategy is established to glycoengineer NK cells. Carbohydrate-based ligands for CD22, a marker for B cell lymphoma, are introduced onto NK cells through either metabolic engineering or glyco-polymer insertion. Such NK cells exhibited greatly enhanced cytotoxicities toward CD22+ lymphoma cells in a CD22-dependent manner. Importantly, both CD22+ lymphoma cell lines and primary lymphoma cells from human cancer patients can be effectively killed by the engineered NK cells. Furthermore, glycoengineered NK cells provided significant protection to tumor-bearing mice. Thus, NK cell glycoengineering is an exciting new approach for cancer treatment complementing the current immune cell genetic engineering strategy. Copyright © 2020 American Chemical Society.