标题：Electrogenerated chemiluminescence sensor for glutathione with Ru(bpy)~(2+)_ 3-doped silica nanoparticles
作者：Shasha Peng;Juan Li;Xiaoli Zhang
作者机构：[Peng, S] School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China;[ Li, J] School of Chemistry and Chemical Engineering 更多
通讯作者地址：[Zhang, XL]Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China.
来源：Journal of solid state electrochemistry
关键词：Reduced glutathione;Electrogenerated chemiluminescence;Ru(bpy)_3~(2+)-doped silica nanoparticles;Sensor
摘要：An electrogenerated chemiluminescence (ECL) sensor for reduced glutathione was developed based on Ru(bpy)_3~(2+)-doped silica nanoparticles-modified gold electrode (Ru-DSNPs/Au). These uniform Ru-DSNPs (about 58+ 4 nm) were prepared by a water-in-oil microemulsion method and characterized by transmission electron microscope and scanning electron microscope. With such a unique immobilization method, a considerable Ru(bpy)_3~(2+) was immobilized three dimensionally on the electrode, which could greatly enhance the ECL response and thus result in an increased sensitivity. The ECL analytical performances of this sensor for reduced glutathione based on the quenched ECL emission of Ru(bpy)_3~(2+) have been investigated in detail. Under the optimum condition, the ECL intensity was linear with the reduced glutathione concentration in the range of 1.0×10~(-9) to 1.0×10~(-4)molL~(-1) (R00.9971). This method has been successfully applied for the determination of reduced glutathione in serum samples with satisfactory results. The as-prepared ECL sensor for the determination of reduced glutathione displayed good sensitivity and stability.