标题：Autophagy inhibition of immature oocytes during vitrification-warming and in vitro mature activates apoptosis via caspase-9 and −12 pathway
作者：Gao H.-H.; Li J.-T.; Liu J.-J.; Yang Q.-A.; Zhang J.-M.
作者机构：[Gao, H.-H] Center for Reproductive Medicine, Hospital for Maternity and Child Care of Linyi City, Linyi, China;[ Li, J.-T] Department of Reproductive 更多
通讯作者地址：[Zhang, J.-M] School of Nursing, Shandong Xiehe UniversityChina;
来源：European Journal of Obstetrics Gynecology and Reproductive Biology
关键词：Apoptosis; Autophagy; Caspase; Immature oocytes; Vitrification
摘要：Objective The aim of this study is to determine the role of autophagy in the immature oocytes during vitrification-warming and in vitro maturation (IVM); the correlations among autophagy, apoptosis, and the activities of caspase in the immature oocytes during vitrification-warming and IVM were also explored. Study design Immature oocytes from mice were vitrified-warmed and IVM. An autophagy inhibitor (3-methyladenine) was supplemented in cryopreservation solutions and warming solutions. The expression of beclin-1 (an autophagy marker), caspase-3, −8, −9, and −12 were measured. Moreover, the viability of vitrified-warmed immature oocytes and their subsequent developmental competence were measured. Results The levels of beclin-1 expression in both mRNA and protein in oocytes experienced vitrification-warming and IVM were significantly higher than that in fresh immature oocytes experienced IVM. The levels of caspase-3, −9, −12 expression in both mRNA and protein in oocytes vitrified with 3-methyladenine were significantly higher than that vitrified without 3-methyladenine. However, the differences in the caspase-8 expression in both mRNA and protein between the oocytes vitrified with 3-methyladenine and that vitrified without 3-methyladenine were not significant. Conclusion Immature oocyte cryopreservation exhibits autophagic activation. Autophagy inhibition of the immature oocytes during vitrification-warming and IVM activates apoptosis via caspase-9 and –12 pathway. © 2017