标题：Comparison of cellular responses induced by low level light in different cell types
作者：Huang, Ying-Ying ;Chen, Aaron C.-H. ;Sharma, Sulbha K. ;Wu, Qiuhe ;Hamblin, Michael R.
通讯作者：Hamblin, M R
作者机构：[Huang, Ying-Ying ;Chen, Aaron C.-H. ;Sharma, Sulbha K. ;Wu, Qiuhe ;Hamblin, Michael R. ] BAR414, Wellman Center for Photomedicine, Massachusetts Gene 更多
会议名称：Mechanisms for Low-Light Therapy V
会议日期：23 January 2010 through 25 January 2010
来源：Progress in Biomedical Optics and Imaging - Proceedings of SPIE
关键词：ATP; Biostimulation; Cytochrome c oxidase; Low level laser therapy; Mitochondria; Nuclear factor kappa B; Reactive oxygen species
摘要：Discoveries are rapidly being made in multiple laboratories that shed "light" on the fundamental molecular and cellular mechanisms underlying the use of low level light therapy (LLLT) in vitro, in animal models and in clinical practice. Increases in cellular levels of respiration, in cytochrome c oxidase activity, in ATP levels and in cyclic AMP have been found. Increased expression of reactive oxygen species and release of nitric oxide have also been shown. In order for these molecular changes to have a major effect on cell behavior, it is likely that various transcription factors will be activated, possibly via different signal transduction pathways. In this report we compare and contrast the effects of LLLT in vitro on murine embryonic fibroblasts, primary cortical neurons, cardiomyocytes and bone-marrow derived dendritic cells. We also examined two human cell lines, HeLa cancer cells and HaCaT keratinocytes. The effects of 810-nm near-infra-red light delivered at low and high fluences were addressed. Reactive oxygen species generation, transcription factor activation and ATP increases are reported. The data has led to the hypothesis that cells with a high level of mitochondrial activity (mitochondrial membrane potential) have a higher response to light than cells with low mitochondrial activity. © 2010 Copyright SPIE - The International Society for Optical Engineering.