标题:Knock down of chitosanase expression in phytopathogenic fungus Fusarium solani and its effect on pathogenicity
作者:Liu, Huaiwei; Zhang, Bo; Li, Changsong; Bao, Xiaoming
作者机构:[Zhang, Bo; Li, Changsong] Shandong Acad Agr Sci, Inst Plant Protect, Jinan 250100, Peoples R China.; [Liu, Huaiwei; Bao, Xiaoming] Shandong Univ, S 更多
通讯作者:Li, CS
通讯作者地址:[Li, CS]Shandong Acad Agr Sci, Inst Plant Protect, Jinan 250100, Peoples R China.
来源:CURRENT GENETICS
出版年:2010
卷:56
期:3
页码:275-281
DOI:10.1007/s00294-010-0299-x
关键词:Chitosanase; Fusarium solani; RNA silencing; Pathogenicity
摘要:Chitosanases are lytic enzymes involved in the degradation of chitosan, a component of fungal cell walls. The phytopathogenic fungus Fusarium solani produces an extracellular chitosanase, CSN1, the role of which in the physiology and virulence of the fungus remains to be expounded. Here, we studied the expression of the CSN1 gene through gene silencing and examined its effect on fungal pathogenicity. A vector construct encoding a hairpin RNA (hpRNA) of CSN1 was constructed and introduced into the F. solani 0114 strain. The results revealed that majority of the transformants exhibited a significant reduction in chitosanase activity compared with the wild-type strain. Further, transformants with silenced CSN1 exhibited no change in mycelial growth and spore formation. However, pea pod and seedling bioassays indicated that transformants with silenced CSN1 were more virulent compared with the wild-type strain, and in sharp contrast to strains in which overexpression of the CSN1 gene resulted in virulence reduction. Although the mechanism remains unclear, our findings did suggest that F. solani chitosanase has a negative effect on fungal pathogenicity.
收录类别:SCOPUS;SCIE
WOS核心被引频次:10
Scopus被引频次:10
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-77953229393&doi=10.1007%2fs00294-010-0299-x&partnerID=40&md5=87744133e8e555b87c3c59ba63a62057
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