标题：A zebrafish intelectin ortholog agglutinates both Gram-negative and Gram-positive bacteria with binding capacity to bacterial polysaccharide
作者：Chen, Lei; Yan, Jie; Sun, Weiping; Zhang, Yan; Sui, Chao; Qi, Jing; Du, Yijun; Feng, Lijun
作者机构：[Chen, Lei; Yan, Jie; Sun, Weiping; Zhang, Yan; Feng, Lijun] Shandong Univ, Shandong Prov Key Lab Anim Cells & Dev Biol, Marine Biotechnol Res Ctr, In 更多
通讯作者地址：[Feng, LJ]Shandong Univ, Shandong Prov Key Lab Anim Cells & Dev Biol, Marine Biotechnol Res Ctr, Inst Dev Biol,Sch Life Sci, Jinan 250100, Shandong, P 更多
来源：FISH & SHELLFISH IMMUNOLOGY
关键词：Innate immunity; Zebrafish intelectin; LPS/PGN; Bacterial agglutination
摘要：Intelectins are glycan-binding lectins found in various species including cephalochordates, urochordates, fish, amphibians and mammals. But their detailed functions are not well studied in zebrafish which is a good model to study native immunity. In this study, we cloned a zebrafish intelectin ortholog, zebrafish intelectin 2 (zITLN2), which contains a conserved fibrinogen-related domain (FReD) in the N-terminus and the unique intelectin domain in the C-terminus. We examined the tissue distribution of zITLN2 in adult zebrafish and found that zITLN2 was expressed in various organs with the highest level in intestine. Like amphioxus intelectins, zITLN2 expression was upregulated in adult zebrafish infected with Staphylococcus aureus with the highest expression level at 12 h after challenge. Recombinant zITLN2 protein expressed in E. coli was able to agglutinate both Gram-negative and Gram-positive bacteria to similar degrees in a calcium-dependent manner. Furthermore, recombinant zITLN2 bound lipopolysaccharide (LPS) and peptidoglycan (PGN) comparably.; Our work on zITLN2 provided further information to understand functions of this new family of lectins and the innate immunity in vertebrates. (C) 2016 Elsevier Ltd. All rights reserved.