标题：Confocal microscopic analysis of the spindle and chromosome configurations of human oocytes matured in vitro
作者：Li, Y; Feng, HL; Cao, YJ; Zheng, GJ; Yang, Y; Mullen, S; Critser, JK; Chen, ZJ
作者机构：[Li, Y; Feng, HL; Cao, YJ; Zheng, GJ; Yang, Y; Mullen, S; Critser, JK; Chen, ZJ]Shandong Univ, Shandong Prov Hosp, Ctr Reprod Med, Jinan 更多
会议名称：60th Annual Meeting of the American-Society-for-Reproductive-Medicine
会议日期：OCT 16-20, 2004
来源：FERTILITY AND STERILITY
关键词：in vitro maturation; human; oocytes; spindle; chromosome
摘要：Objective: To assess the potential effects of in vitro maturation (IVM) of human oocytes on the meiotic spindle and associated chromosome configuration.; Design: Prospective study. Setting: Hospital-based IVF center.; Patient(s): Patients with polycystic ovary syndrome (PCOS) undergoing unstimulated and stimulated cycles of oocyte retrieval.; Intervention(s): Immature (germinal vesicle and metaphase I) and mature (metaphase II) oocytes were collected from PCOS patients. The meiotic spindle and chromosome configurations in oocytes matured in vitro and in vivo were studied by confocal microscopy, With fluorescent labeling techniques for visualization of both microtubules and chromatin.; Main Outcome Measure(s): Meiotic spindle and associated chromosome configurations.; Result(s): Oocytes can develop to the metaphase It stage after IVM. Confocal microscopic observations revealed that the oocytes matured in vitro had a higher frequency of abnormal meiotic spindle and chromosomal alignment morphology than in vivo-matured oocytes. These abnormalities included a partial or total disorganization of the meiotic spindle inicrotubules. Abnormal chromosome organization included dispersal of chromosomes or chromosomes with an aberrant, less-condensed appearance. The proportions of abnormality in spindle and chromosome configurations in oocytes matured in vitro were 43.7% and 33.3%, respectively, which was significantly higher than in those oocytes matured in vivo (13.6% and 9.1%).; Conclusion(s): In vitro maturation can have deleterious effects on the organization of the meiotic spindle and chromosome alignment of human oocytes. This result suggests one possible explanation for the reduced developmental potential of oocytes matured in vitro compared with those matured in vivo. This is likely a contributing factor to the overall lower clinical outcomes observed after IVM and ET.