标题：Soluble mediators released from PI-IBS patients\' colon induced alteration of mast cell: Involvement of reactive oxygen species
作者机构：[Han, W] Department of Gastroenterology, Qilu Hospital, Shandong University, Jinan, Shandong Province, China;[ Lu, X] Department of Gastroenterology, 更多
通讯作者地址：[Guo, CH]Shandong Univ, Sch Med, Dept Pathol, 44 Wenhua W Rd, Jinan 250100, Shandong, Peoples R China.
来源：Digestive Diseases and Sciences
关键词：Inflammation;Irritable bowel syndrome;Mast cells;Protease activated receptor 2;Reactive oxygen species
摘要：Background: Growing evidence suggests that patients with post-infectious irritable bowel syndrome (PI-IBS) have increased mast cell activation, and that mucosal soluble mediators are involved in the pathophysiology of visceral hyperalgesia. In addition, previous findings show that reactive oxygen species (ROS) and protease-activated receptors (PARs) are mediators of persistent hyperalgesia. Aims: This article aims to investigate: (1) the ability of soluble factors from colonic biopsies to active peritoneal mast cells (PMCs) in vitro; (2) whether the effects of PMCs degranulation induced by soluble mediators are related to PARs activation; and (3) the ability of phenyl N-tert-butylnitrone (PBN), a ROS scavenger, to modify these alterations. Methods: Supernatant (SUP) from colonic biopsies was collected and applied to PMCs for 12 h. Activation of PMCs was evaluated. The expression of PAR 2 in PMCs was examined by RT-PCR and double-immunofluorescence staining. PBN (10 mM) treatment was administered, then previous alterations were observed again. Results: Stimulation with SUP of PI-IBS led to an increase in activation of PMCs. PAR 2mRNA expression was significantly increased in PMCs induced by SUP of PI-IBS compared to healthy subjects. After being treated by PBN, the SUP-induced enhancement of PMCs activities could be weakened, and PAR 2mRNA expression was significantly decreased. A similar result of immunoreactivity for PAR 2 was observed in PMCs. Conclusions: The study shows that ROS scavenger reverses the SUP of PI-IBS-induced enhancement of PMCs activities, and that these effects may be related to activation of PAR 2. These findings might pave the way to new therapeutic targets in PI-IBS.