标题:T7 exonuclease-assisted and target-triggered cascade dual recycling signal amplification strategy for the sensitive and specific detection of adenosine
作者:Wang, Gang; Wang, Lei; Li, Xia; Xu, Xiaowen; Jiang, Wei
作者机构:[Wang, Gang; Wang, Lei; Jiang, Wei] Shandong Univ, Sch Pharmaceut Sci, Jinan 250012, Shandong, Peoples R China.; [Li, Xia] Liaocheng Univ, Dept Chem 更多
通讯作者:Jiang, Wei;Jiang, W
通讯作者地址:[Jiang, W]Shandong Univ, Sch Pharmaceut Sci, Jinan 250012, Shandong, Peoples R China.
来源:TALANTA
出版年:2019
卷:197
页码:234-238
DOI:10.1016/j.talanta.2019.01.020
关键词:T7-exonuclease; Cascade amplification; Dual recycling
摘要:Adenosine is closely related to the development of cancer, and it can be regarded as a biomarker for cancer diagnosis and therapy. Here, a T7 exonuclease (T7 Exo)-assisted and target-triggered cascade dual recycling signal amplification strategy was developed for the sensitive and specific detection of adenosine. In this strategy, a capture strand (Cap)-inhibit strand (Inh) duplex and a DNA hairpin with intact G-quadruplex sequence are designed. In the presence of adenosine, Cap binds with adenosine specifically to form Cap-adenosine complex with recessed 5'-hydroxyl termini, causing the release of Inh. Under the action of T7 Exo, the adenosine is released from Cap-adenosine complex. Then the released adenosine interacts with the next Cap-Inh duplex to promote the release of a new Inh and the recycle I is completed. After that, the released Inh hybridizes with hairpin to form Inh-hairpin duplex with blunt 5'-hydroxyl termini. Under the same action of T7 Exo, the Inh gets released and a G-quadruplex sequence is obtained. Subsequently, the released Inh continues opening hairpin and the recycle II is accomplished. Finally, plenty of G-quadruplex sequences are generated and then interact with Nmethyl-mesoporphyrin IX (NMM) to obtain enhanced fluorescence signal. The limit of detection (LOD) of the proposed strategy is estimated to be 9.8 x 10(-7) mol L-1, and the linear range of the strategy is from 5.0 x 10(-6) mol L-1 to 7.0 x 10(-4) mol L-1. Besides, the proposed strategy is capable of distinguishing adenosine from its analogues. This strategy holds promise in adenosine related biomedical research, clinical diagnosis and disease treatment.
收录类别:EI;SCOPUS;SCIE
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85059861115&doi=10.1016%2fj.talanta.2019.01.020&partnerID=40&md5=e2f2aeda0dddf821012d334354d0c43d
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