标题：Expression, purification and preliminary crystallographic analysis of a haem-utilizing protein, HutX, from Vibrio cholerae
作者：Su, Tiantian;Chi, Kaikai;Wang, Kang;Guo, Liming;Huang, Yan
作者机构：Shandong Univ, Sch Life Sci, State Key Lab Microbial Technol, Jinan 250100, Shandong, Peoples R China;Shandong Univ, Sch Life Sc
来源：Acta crystallographica. Section F, Structural biology communications
关键词：haem uptake;iron acquisition;Vibrio cholerae
摘要：Vibrio cholerae, the causative agent of cholera, has developed a variety of mechanisms to obtain the limited-availability iron from human hosts. One important method for iron acquisition is through haem-uptake systems. Although the transport of haem has been widely studied, the fate of haem once it enters the cytoplasm remains an open question. Here, preliminary X-ray crystallographic analysis was performed on HutX, a member of the conserved haem-utilization operon from V. cholerae strain N16961. The crystals of HutX were found to belong to the orthorhombic space group C222(1), with unit-cell parameters a = 50.1, b = 169.0, c = 81.8 angstrom. There are two protein molecules in the asymmetric unit, with a corresponding Matthews coefficient V-M of 2.06 angstrom(3) Da(-1) and a solvent content of 40.28%.